植物生长调节剂对线艺春兰根状茎的增殖与分化的影响

Effects of Plant Growth Regulators on the Rhizome's Multiplication and Differentiation in Cymbidium goeringii with Verge Line Pattern Leaves

以线艺春兰‘绿云’的类原球茎为材料，研究了植物生长调节剂等因素对根状茎的增殖与分化的影响。结果表明：长期继代培养及保存宜选用1／2MS固体培养基。细胞分裂素6-BA浓度为1．0mg· L^-1时根状茎的增殖效果最佳。0～3．0mg· L^-1范围内IBA促进根状茎的生长较NAA效果好。1／2MS＋6-BA1．0mg· L^-1＋IBA1．0mg· L^-1培养基最利于根状茎的分化，芽分化率达到36．9％。培养基添加芋艿糊可显著促进根状茎的增殖与生长。根状茎分割以掰开方式且接种团块带有3～5条根状茎较适宜。线艺春兰叶片上的腹轮艺通过组培可以稳定遗传。

The rapid multiplication system of Cymbidium goeringii ‘Lüyun＇ with verge line pattern leaves by protocorm like-body was established. Effects of plant growth regulators on multiplication and differentiation of rhizomes were studied. 1/2 MS solid medium was suitable for subculture and preservation of the rhizome. The optimal medium for multiplication and growth of rhizomes was MS ＋ 6-BA 1.0 mg · L^-1. IBA was more effective to the growth of rhizomes than NAA with increasing of auxin concentration （0 - 3 mg · L^-1 ）. The optimum medium for differentiation of rhizomes was 1/2 MS supplemented with 6-BA 1.0 mg · L^-1 and IBA 1.0 mg · L^-1 , which regeneration rate of shoots was as high as 36. 9%. Dasheen paste was better to the multiplication of rhizomes than coconut milk and banana mud added to the culture medium. Single conglomeration with 3 - 5 rhizomes was optimum by the way of broking off with fingers and thumb. Verge line pattern on the leaf could be stably inherited.