平阳霉素乳剂对增生期毛细血管瘤细胞凋亡的诱导

Pingyangmycin emulsion inducing apoptosis in infantile proliferating capillary hemangiomas

目的探讨平阳霉素乳剂对增生期毛细血管瘤细胞凋亡的影响,寻找促使血管瘤消退的新方法。方法选取6个月以内体表增生期毛细血管瘤志愿患儿30例,随机分为两组,每组15例。将平阳霉素乳剂涂于用药组瘤体表面;对照组则只涂以基质。用药7d后,两组均手术切除瘤体,制成石蜡切片观察细胞微观变化;采用TUNEL方法检测两组细胞凋亡指数;另取两组各2例制成电镜标本观察细胞超微结构变化。结果用药组比对照组凋亡细胞明显增多,两组凋亡指数比较差异有统计学意义(t=6.893,P<0.01)。结论平阳霉素乳剂可诱导增生期毛细血管瘤细胞发生凋亡而促进其消退,其机制可能与阻断细胞周期和激活凋亡信号传导通路有关。

Objective To insight whether PYM emulsion induces apoptosis more rapidly in proliferating capillary hemangiomas so as to find the optimal method for hemangiomas＇ treatment. Methods Thirty volunteers of infantile proliferating hemangiomas were divided into control group （15 cases） and medicational group（ 15 cases）. PYM was made into emulsion and smeared on the surfaces of the leision in medicational group with 3 times every day as well as only matrix in control group. The specimens were resected on day 7,then made into pathological slices and electron microscope slices in order to observe the cells microcosmic structure changes and uhrastructure changes. Furthermore , the apoptotic index of two groups were detected by the molecular biology method （TUNEL test ）. Results The number of apoptotic cells were lower in control group （AI 9.693 ± 4.948） but higher apparently in medicational group （AI 39.373±15.927） . The difference between two groups was significant（ t = 6.893, P 〈 0.01 ） . Conclusions PYM emulsion can effectively accelerate apoptosis in infantile proliferating capillary hemangiomas .The mechanism is supposed to related to the blockage of cell cycle and activation of apoptotic signal transduction pathway.