神经生长因子与睫状神经营养因子促进感觉和运动纤维再生的差异性研究

The difference of nerve growth factor and ciliary neurotrophic factor between the sensitive and motor fibres regeneration

目的探讨神经生长因子(NGF)与睫状神经营养因子(CNTF)对不同类型神经纤维再生的促进作用。方法利用梭形双通道桥接管桥接32只SD大鼠坐骨神经10mm缺损。将动物随机分为两组,A组:两支管内均加入医用几丁糖凝胶;B组:两支管内加入医用几丁糖凝胶后分别注入NGF和CNTF。术后12、16周行电生理检测和组织化学染色。结果Holmes银染示B组CNTF侧再生纤维均较NGF侧再生神经外膜薄,纤维排列整齐,粗细均匀;硫代乙酰化胆碱染色示B组CNTF侧呈棕红色的阳性纤维较NGF侧数目较多,粗细均匀,排列整齐;而碳酸酐酶染色则显示B组NGF侧呈褐色的阳性神经纤维密度及排列稍较CNTF多。电生理检测见NGF侧再生神经复合肌肉动作电位(CMAP)和皮层体感诱发电位(CSEP)的潜伏期较CNTF侧长,而波幅较低(P<0.05)。结论CNTF促进运动纤维再生的相对作用较强,而NGF具有相对较强的促进感觉纤维再生的作用。

Objective To investigate the effects of nerve growth factor（NGF） and ciliary neurotrophic factor（CNTF） to promoting the sensitive and motor fibres regeneration. Methods Thirty two Sprague-Dawley rats with 10mm gap of sciatic nerve were operated and bridged with the new double channel nerve conduit in rhomboid shape. They were randomly divided into two groups, with each in twelve animals. In the first group, 200μl of chitin for medical use was injected into the conduit, and in the second group the two branches of the conduit contained 100μl of the chitin and 5μl NGF or CNTF. At twelve and sixteen weeks after the operation, we evaluated the sensitive and motor fibres of regenerative nerve with Holmes, Acetylcholinesterase and Carbonic anhydrase staining. Results After the twelve and sixteen weeks of the operation, there were not significant differences of the regenerative nerve fibres between two channels in the first groups. But in the second group, the number of the motor fibres of the regenerative nerve of CNTF branch channel was much more and distributed better than the others, while the number and density of sensitive fibres was inferior to NGF. The latency of compound muscle active potential and cortical somatosensory evoked potential of the nerve in the CNTF branch was shorter than the one in the NGF branch, but its amplitude was higher. Conclusions The CNTF could significantly promote the regeneration of motor fibres and the NGF could promote better for the regeneration of sensitive fibres.