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T-DNA插入稻瘟病菌Gγ亚基基因启动子的突变体A1-412丧失形成附着胞、穿透和致病能力

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摘要 用TAIL-PCR的方法获得了A1-412突变体的T-DNA侧翼基因组序列,测序结果表明,外源T-DNA插入到稻瘟病菌G蛋白γ亚基基因MGG1(MagnaporthegriseaGproteinGamma亚基)的启动子区域.MGG1编码93个氨基酸,具有典型的G蛋白γ亚基结构域(GGL)和C末端CAAX框,并与其他丝状真菌G蛋白γ亚基有较高的一致性.外加cAMP能诱导部分A1-412分生孢子形成附着胞,但这些附着胞的形态不正常,不能穿透洋葱内表皮或水稻叶片.另外,A1-412与相对应交配型的菌株杂交时几乎不产生子囊壳.将MGG1基因重新导入A1-412突变体可部分恢复上述表型.这些结果表明,G蛋白γ亚基MGG1可能涉及稻瘟病菌形态分化、有性生殖、致病性等方面的调节作用.
作者 梁慎 王政逸 刘朋娟 李德葆
机构地区 浙江大学生物技术研究所
出处 《科学通报》 EI CAS CSCD 北大核心 2006年第17期2037-2040,共4页 Chinese Science Bulletin
基金 国家重点基础研究发展计划(批准号:2006CB101901) 国家自然科学基金(批准号:30570054,30270861)资助项目.
关键词 稻瘟病菌 G蛋白γ亚基基因MGG1 形态分化 致病性
分类号 S435.111.41 [农业科学—农业昆虫与害虫防治]
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参考文献11

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科学通报
2006年 第17期

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