慢性病毒性肝炎小鼠动物模型的建立及其特征分析

Establishment and Characterization of a Chronic Viral Hepatitis Model Induced by Murine Hepatitis Virus Type 3

慢性病毒型肝炎是一种严重危害人类健康的常见病,多发病,目前尚缺乏理想的实验动物模型来阐明其具体的发病机制,从而使得对其防治的研究受到限制。本研究采用纯化的3型鼠肝炎病毒(MHV-3)经腹腔注入近交系C3H/HeJ小鼠体内,小鼠感染MHV-3后,约63%存活,存活的小鼠一般情况较正常对照组差,肝脏组织呈现持续炎性改变,血清ALT、AST升高而TP、ALB有所下降,与人类慢性病毒性肝炎的发生发展极为相似,可作为研究慢性病毒性肝炎的比较理想动物模型。

Approximately 8% of the population in China are carriers of HBV and about 5% have HCV infection. The underlying mechanisms of pathogenethis of the establishment of a chronic infection with HBV and HCV are not fully understood. Since there is no ideal model to explain chronic HBV and HCV infection, we have decided to attempt to establish and characterize a substitute model in C3H/HeJ mice induced by murine hepatitis viral type 3 （MHV-3）. C3H/HeJ 6-8weeks old mice received 10pfu of MHV-3 intraperitoneally and 63% of them developed a chronic course of virus infection characterized by persistence of lymphocyte infiltration and macrophage activation until 40 days post infection. The mice underwent fluctuated serum ALT, AST levels and a decreased TP, ALB level. We conclude that this substitute model could be applied in studies towards understanding the mechanism of chronic viral hepatitis.