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口蹄疫病毒P1+2A基因在BHK–21细胞中的表达 被引量：1

Expression of FMDV P1+2A Gene in BHK-21 Cells
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摘要 本研究将已构建好的、包含口蹄疫病毒P1+2A基因的重组表达质粒pQE-Tri/P1+2A经质脂体2000转染哺乳动物细胞BHK-21,转染后一定时间进行检测。通过SDS-PAGE、Western-blotting、荧光抗体染色、ELISA等检测方法表明,FMDVP1+2A基因片段在BHK-21细胞中成功表达,表达的蛋白能被口蹄疫阳性血清所识别而且具有良好的生物学活性。这一结果的取得,为进一步研制新型口蹄疫基因工疫苗及其配套诊断试剂奠定了基础。 In this study, recombinant expression plasmid pQE-Tri/P1＋2A was constructed and then transfected into BHK-21 cell line mediated by liposome 2000. The results of SDS-PAGE, Western-blotting, fluorescence antibody and ELISA showed that FMDV P 1 ＋2A protein was expressed in BHK-21 cell line successfully and can be recognized by FMD positive serum. The result will lay a foundation for study of FMDV gene engineered vaccines as well as the development of diagnostic reagents.
作者 龚真莉 刘湘涛 田宏 吴锦艳 代兴国 尚佑军 陈国栋
机构地区 中国农业科学院兰州兽医研究所农业部畜禽病毒学重点实验室
出处 《中国病毒学》 CSCD 2006年第5期454-457,共4页 Virologica Sinica
基金 国家863高技术研究发展计划(2003AA241110)
关键词 口蹄疫病毒 P1+2A基因 表达载体pQE-Trisystem BHK-21细胞转染 Foot-and-mouth Disease Virus P1＋2A gene Expression vector pQE-Trisystem BHK-21 Cells transfection
分类号 S852.65 [农业科学—基础兽医学]
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参考文献12

  • 1Xie Q G(谢庆阁).Foot-and-Mouth Disease (口蹄疫)[M].Beijin:Chinese Agricultural Publishing Company,2004.
  • 2Yin Z,Liu J H (殷震,刘景华).Animal Virology (动物病毒学)[M].Beijin:Science Press,1985,395-415.
  • 3林瑞庆,罗满林,黄毓茂,刘镇明,辛朝安.O型口蹄疫病毒VP1基因的克隆与表达[J].华南农业大学学报,2004,25(1):92-95. 被引量：6
  • 4Brown F.New approaches to vaccination against foot-and-mouth disease[J].Vaccine,1992,10:1022～1026.
  • 5Beck,E,Strohmaier K.Subtyping of European foot-and-mouth disease virus strains by nucleotide sequence determination[J].J Virol,1987,61:1621-1629.
  • 6King A M Q,Underwood B O,et al.Biochemical identification of viruses causing the 1981 outbreaks of foot-and-mouth disease in the UK[J].Nature,1981,293:479-480.
  • 7冷青文,郭慧琛,云涛,刘在新,谢庆阁,张居农.口蹄疫病毒多基因片段的克隆及其杆状病毒表达载体的构建[J].中国兽医科技,2004,34(6):16-20. 被引量：10
  • 8Jarasvech C,Clayton B,Peter W.M,et al.Antibody response in mice inoculated with DNA expressing foot-and-mouth disease virus capsid proteins[J].J Virol,1998:4454-4457.
  • 9龚真莉,刘湘涛,刘磊,尚佑军,尹双辉,田宏,郑海学,陈国栋.口蹄疫病毒P1+2A基因真核表达载体的构建[J].甘肃农业大学学报,2006,41(1):8-11. 被引量：10
  • 10Bachrach H L.Foot-and-mouth disease virus[J].Annu Rev Microbiol,1968,22:201-244.

二级参考文献32

  • 1吴锦艳,刘湘涛,胡永浩,尚佑军,田宏,孙世琪,尹双辉,冯霞.猪口蹄疫病毒对三种动物细胞嗜性的研究[J].甘肃农业大学学报,2005,40(3):306-310. 被引量：8
  • 2杨永钦,王家驹.口蹄疫基因工程疫苗研究进展[J].云南畜牧兽医,1997(1):27-29. 被引量：6
  • 3BROWN F. Vaccination against foot and mouth disease virus[J]. Vaccine, 1992,10:1 022- 1 026.
  • 4DIMARCHI R, BROOKE G, GALE C, et al. Characterizing sequence variation in the VP1 capsld proteins of foot and mouth disease virus(serotype O) with respect to virion structure [J]. Science, 1986,232:639 - 642.
  • 5CHINSANGARAM J, BEARD C, MASON P W, et al.Antibody responses in mice inoculated with DNA expressing foot-and-mouth disease virus capsid proteins [J]. Journal of Virology, 1998,72:4 454- 4 457.
  • 6WARD G, RIEDER E, MASON P W. Plassmid DNA encoding replication foot-and-mouth disease virus genomes induces antiviral immune responses in swine[ J]. Journal of Virology,1997,71:7 442 - 7 447.
  • 7CARRILLO C, WIGDOROVITZ A, OLIVEROS J C, et al.Protective immune response to foot-mid-mouth disease virus with VP1 expressed in transgenic plants[J]. Journal of Virology,1998,2:1 688- 1 690.
  • 8LUBRUTH J, BROWN F. Identification of native foot-and-mouth disease virus non-structural protein 2C as a serological indicator to differentiate indicator from vaccinated livestock[J]. RES Vet Science, 1995,59:70- 78.
  • 9SURYANARAYANA V V S, VISWANATHAN S, RATISH G, et al. E coli expressed proteins as diagnostic reagents for typing of foot-and-mouth disease virus [ J].Arch Virol.1999,144:1 701- 1 712.
  • 10Kitching R P.Foot-and-mouth disease: Current world situation [J].Vaccine, 1999, 17:1772-1774.

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中国病毒学
2006年 第5期

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