血管内照射对兔血管平滑肌细胞增殖和凋亡的影响

EFFECTS OF ^(188)Re IRRADIATION ON INHIBITION PROLIFERATION AND APOPTPSIS OF RABBIT VESSEL SMOOTH MUSCLE CELLS

目的:探讨188铼(188Re)血管内照射对血管平滑肌细胞增殖和凋亡的影响。方法:40只新西兰白兔随机分为对照组(n=20只)和照射组(n=20只),均行腹主动脉球囊内皮拉伤术。照射组内皮拉伤后行188Re血管内照射治疗,管腔下0.5 mm处累计吸收剂量为15 Gy;对照组则不行血管内照射。分别于术后1、3周处死动物,取病理组织学标本进行增殖细胞核抗原(PCNA)和凋亡细胞分析。结果:照射组第1、3周PCNA细胞阳性率分别为(27.69±7.04)%与(20.88±4.55)%,明显低于对照组(42.71±6.11)%(、29.48±10.13)%(P均<0.05);照射组第3周平滑肌细胞凋亡细胞百分比为(40.16±7.93)%,明显高于对照组(28.50±9.39)%(P=0.018)。结论:188Re血管内照射能抑制血管平滑肌细胞增殖,促进血管平滑肌细胞凋亡,从而抑制新生内膜增生,减少PTCA术后再狭窄的发生。

Objective：To evaluate the effect of beta-irradiation （^188Re） intravascular irradiation on inhibiting proliferation and apoptosis of rabbit vessel smooth muscle cells after balloon angioplasty. Methods： Forty New Zealand white rabbits were divided into two groups randomly： the control group （ n =20） and the irradiation group（ n = 20）. In the control group, balloon catheters were overstretched on abdominal aorta artery to denude endothelium. In the irradiation group, radioactive liquid filled balloon irradiation was performed in the target segment immediately after balloon overstretched injury until the accumulative radioactive dose was added up to 15 Gy. Target segments were harvested at 1,3 weeks after the operation respectively. Patholohistological samples were analyzed. Result：In the 1st week and the 3rd weeks, PCNA positive cells were decreased significantly in the irradiation group than that in the control group [（27.69 ±7.04）% vs （42.71±6. 11）%, P =0.0004;（20. 88±4.55）% vs （29.48±10. 13）%, P =0.046]. In 3rd week, TUNEL positive cells were increased significantly in the irradiation group than that in the control group [（40. 165±7.93）% vs （28.50±9.39）%, P =0. 018]. Conclusion：Applying ^188Re to intraluminal irradiation can inhibit the proliferation and accelerate the apoptosis of VSMCs. Intravascular irradiation therapies can prevent recurrence of restnosis after angioplasty efficiently.