摘要
利用基于统计学的实验设计RSM(Responsesurfacemethodology)优化了BurkholderiacepaciaCF-66产新型抗菌活性物质CF66I的发酵培养基组成。首先,用部分重复因子实验对培养基组分NH4Cl,MgSO4·7H2O,柠檬酸钠及酵母粉浓度对菌株产CF66I的影响进行评价,找出主要影响因子为柠檬酸钠和酵母粉。两者均为正影响,其他组分对CF66I活性的影响不显著。其次用最陡爬坡路径逼近最大响应区域。最后用中心组合设计及响应面分析确定主要影响因子的最佳浓度。菌株在优化培养基中培养较初始培养基CF66I活性提高了约两倍。
Statistics-based experimental design was used to investigate the effect of medium components on CF66I production by Burkholderia cepacia CF-66. A fractional factorial design augmented with center points revealed that sodium citrate and yeast extract were the most significant factors, which influenced CF66I production positively, while the other factors were not important with the levels tested. The method of steepest ascent was used to approach the proximity of optimization. This task was followed by a central composite design to determine the optional concentration of sodium citrate and yeast extract. The optimized medium allowed the activity of CF66I to be increased from 2.12 U/ml to 6.24 U/ml,about 200% higher than the original medium.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2006年第9期56-60,共5页
China Biotechnology
基金
辽宁省自然科学基金资助项目(12040949)
