超声检查和血管造影对兔动脉粥样硬化狭窄模型的评价

Evaluating the degree of atherosclerotic stenosis models in rabbits by using ultrasonographic image and angiography

目的:复制动脉粥样硬化狭窄的模型,比较超声和血管造影测量动脉狭窄程度的一致性。方法:实验于2003-02/05在河南省人民医院心血管内科实验室完成。选择雄性4月龄新西兰大白兔10只,分笼饲养,饲喂正常的全价颗粒饲料,自由饮水,观察1周后进入实验。1周后开始高脂饲料(含1.5%胆固醇)喂养,高脂喂养3d后用股动脉穿刺法行腹主动脉内皮剥脱术。术后继续喂养同样的高脂饲料。于术后第9周行腹主动脉超声和血管造影检查,记录腹主动脉狭窄程度。结果:纳入动物10只,均进入结果分析。①超声检查:术后第9周均可见腹主动脉有不同程度的粥样硬化斑块和狭窄,测量狭窄程度在46%～68%[(53.970±6.136)%],狭窄长度在6～20mm。②血管造影:术后第9周显示所有兔的动脉粥样硬化狭窄均形成,目测狭窄程度在50%～70%[(57.000±7.888)%],狭窄长度8～23mm。内皮剥脱术后第9周腹主动脉超声检查与血管造影检查的狭窄程度比较差异无显著性(P>0.05)。结论:动脉内膜剥脱术同时饲喂高胆固醇饮食可以在短时间内复制出动脉粥样硬化狭窄模型,超声和血管造影均能准确判定血管狭窄程度。

AIM： To establish models of atherosclerotic stenosis of rabbits, and compare the consistency of evaluating the degree of stenosis by ultrasonographic image and angiography. METHODS： The experiment was carried out in the laboratory of Department of Cardiovascular Medicine, Henan Provincial People＇s Hospital from February to May 2003. Ten male New Zealand rabbits aged 4 months were fed with a standard diet in separate cages for 1 week without inhibiting drinking. One week later, these rabbits were fed with cholesterol-supplemented diet （1.5% cholesterol） and were subject to balloon catheter injury of the abdominal aorta by puncturing into femoral artery after 3 days of cholesterol-fed. Rabbits were continuing fed with cholesterol-rich diet after the operation. Ultrasonographic image and angiography were used to record the degree of stenosis of the abdominal aorta at the 9^th week after operation. RESULTS： All the 10 rabbits entered the result analysis. （1） Ultrasonographic image： Different degrees of atherosclerotic plaque stenosis of abdominal aorta could be detected by uhrasonographic image at the 9^th week after balloon denudation. The rate of stenosis was from 46% to 68% [（53.970±6.136）%] and it was 6-20 mm in length. （2）Angiography： Stenosis of abdominal aorta was observed at the 9^th week by angiography and the rate of stenosis was 50%-70% [（57.000±7.888）%] and it was 8-23 mm in length. There was no significant difference in the degree of stenosis detected by ultrasonographic image or angiography at the 9^th week after balloon denudation （P 〉 0.05）. CONCLUSION： The animal model of atherosclerotic stenosis can be successfully established in a short time by balloon endothelial denudation of abdominal aorta combined with cholesterol-feed, Both ultrasonography and angiography can accurately measure the degree of stenosis of the abdominal aorta.