膜型-Ⅰ型基质金属蛋白酶mRNA在人喉鳞癌中的表达及临床意义

Membrane type-Ⅰmatrix metalloproteinase expression and clinical significance in human laryngeal squamous cell carcinoma

目的:为了提高喉鳞状细胞癌病人的生存质量与生存率,降低致残率,减少过度治疗,寻找适当的能反映实际肿瘤浸润转移的肿瘤标志物。在转录水平研究能够降解细胞外基质的膜型-Ⅰ型基质金属蛋白酶mRNA在人喉鳞癌中定位及其定量表达的临床意义。方法:采用原位杂交法对50例人喉鳞癌原发灶中的膜型-Ⅰ型基质金属蛋白酶mRNA进行检测,采用图像分析对其进行定量分析,并分析其与肿瘤的分期、分级、淋巴结转移等临床病理参数的关系。结果:原发灶中MT1-MMP mRNA主要在肿瘤细胞的胞浆中呈强阳性表达,进展期肿瘤的癌巢周边的基质细胞中呈弱阳性表达。MT1-MMP mRNA的表达与肿瘤的分级、淋巴结的转移、术后的复发有关(P=0.000);对有淋巴结转移的正确预测值为95.5%,无淋巴结转移的正确预测值为60.7%。结论:MT1-MMP mRNA主要在肿瘤细胞的胞浆中表达,其高表达与喉癌的浸润转移有关,是预测淋巴结转移的一个较好指标。

Objective：To order to improve the quality of life and survival rate of laryngeal squamous cell carcinoma patients, decrease the mutilation rate and over -treatment, and look for suitable tumor markers that could reflect the actual laryngeal carcinoma invasion and metastasis, We studied the localization of membrane type I matrix metalloproteinase（ MT1 -MMP） that can degrade cell matrix at the transcription level in human laryngeal squamous cell carcinoma, and the clinical significance of it＇s expression. Methods： MT1 - MMP mRNA of 50 primary laryngeal carcinoma tissues were detected by in situ hybridization, quantitatively analyzed their expression by image analysis system, and the relation to T stage, lymph node metastasis, pathology grade. Results： MT1 - MMP mRNA localized predominantly in carcinoma cells cytoplasm with relatively weak stained in the stromal cells around carcinoma nest. The expression correlated with neoplasm pathology grade, lymph node metastasis and post - operation recurrence （ P = 0. 000）. It was valuable to predict lymph node metastasis, the right prediction ratios to pN ^＋ and pN^- were 95.5 % and 60.7% respectively. Conchlsions：MT1 -MMP mRNA is expressed predominantly in carcinoma cells cytoplasm, it＇s high expression correlates with lymph node metastasis and neoplasm recurrence. It is one of good markers to predict neoplasm invasion and metastasis.