结肠癌中热休克蛋白70和糖调节蛋白94的表达与临床病理的关系

Correlation between clinicopathology and expression of heat shock protein in human colonic adenocarcinoma

目的:探讨热休克蛋白70(HSP70)和糖调节蛋白94(grp94)在人结肠癌组织中的表达与临床病理的关系。方法:应用免疫组织化学和病理学图像分析方法研究80例人结肠癌组织及其癌旁组织、发生及未发生转移的癌组织中HSP70和grp94的表达。结果:在结肠癌组织中HSP70和grp94的表达明显高于癌旁组织(92.5%,85.0%VS56.3%,42.5%,P<0.01)。HSP70和grp94在中度和低度分化的结肠癌组织中的表达率明显高于癌旁组织(93.7%,87.5%;100%,90.0%VS56.3%,42.5%;P<0.01)。HSP70和grp94在DukesC期(97.1%,91.2%)和D分期(100%,90.9%)的表达比DukesA期(80.0%,70.0%)和B期(78.6%,71.4%)的癌组织阳性表达率高(P<0.05)。结论:HSP70和grp94在发生转移的低分化结肠癌组织和未发生转移的高分化癌组织中表达存在明显不同,它们的高表达可以作为结肠癌的诊断或预后指标。

Objective：To investigate the correlation between prognosis and expression of heat shock protein 70 （HSP70） and glucose - regulated protein 94 （ grp94 ） in human colonic carcinoma. Methods： The expression of HSP70 and grp94 was studied in 80 human colonic cancers with or without metastasis as well as in their adjacent mucous membrane by means of immunohistochemistry and pathology photograph analysis. Results： The expression of HSP70 and grp94 was significantly higher in cancer than in adjacent mucous membrane （92.5% , 85.0% vs 56.3% , 42.5% , P 〈 0.01 ）. HSP70 and grp94 expressed higher in moderately and poorly differentiated colonic cancers than in their adjacent tissues （93.7% , 87.5 % ; 100% , 90.0% vs 56.3% , 42.5 % ; P 〈 0.01 ）. Dukes C and D stages of colonic cancers showed higher positive rates than Dukes A and B stage groups （97.1% , 91.2% ; 100%, 90.9% ; vs 80.0%, 70.0% ; 78.6%, 71.4% ; P 〈 0.05 ）. Conclusion： The HSP70 and grp94 expression in poorly differentiated colonic cancers with metastasis is significantly higher than that in well differentiated cancers without metastasis. The overexpression of HSP70 and grp94 can be used as diagnostic or prognostic markers for colonic cancer.