GABA对胰腺癌细胞株SW1990生长的影响

Effect of gamma-aminobutyric acid on the growth of pancreatic cancer cell line SW1990

目的:观察γ-氨基丁酸(GABA)对胰腺癌SW1990细胞生长的影响．方法:采用MTT法和流式细胞术检测GABA对胰腺癌SW1990细胞增殖、细胞凋亡、细胞周期的影响,放射免疫分析法检测cAMP含量．结果:不同浓度的GABA(20-320μmol／L)促进胰腺癌SW1990细胞的生长,促进细胞周期比例的分布,使G0／G1细胞减少,S期和G2／M细胞增多,同时促进胰腺癌SW1990细胞内cAMP含量的增加,且呈剂量依赖性(P<0．01)．显著抑制细胞凋亡,凋亡率由27．4％降低到5．4％(X2=10．19,P<0．01)．结论:GABA促进胰腺癌SW1990细胞生长,可能通过受体后信息介导．

AIM：To investigate the effect of gammaaminobutyric acid （GABA） on the growth of pancreatic cancer cell line SW1990. METHODS： Pancreatic cancer cell line SW1990 was cultured by routine method, and then treated with different concentrations of GABA （20-320 μmol/L）. The proliferation, apoptosis, and cell cycle of SW1990 cells was investigated by MTT assay and flow cytometry, respectively. Radioimmunoassay was used to measure the intracellular cyclic adenosine monophosphate （cAMP） content. RESULTS： The different concentrations of GABA promoted the growth of SW1990 cells and affected the distribution of cell cycle. The percentage of cells in G0/G1 phase was decreased while that in S and G2/M phase was increased, The content of intracellular cAMP was increased with the increase of GABA concentration in a dose-dependent manner （P 〈 0.01）. The apoptosis rate of SW1990 cells was decreased from 27.5% to 5.4%, which had significant difference （9（2 = 10.19, P 〈0.01）. CONCLUSION： GABA can promote the proliferation of SW1990 cells by inhibiting apoptosis and influencing the distribution of cell cycle, which may be mediated by the information transition of post-receptor.