兔实验性上颌窦炎黏膜纤毛运动的检测

Observation of cilia movement in rabbits with experimental maxillary sinusitis

目的:利用兔实验性上颌窦炎模型观察手术前后上颌窦黏膜纤毛运动的变化。方法:将40只新西兰大白兔分成正常对照(35侧)、鼻窦炎(50侧)和鼻窦炎术后(40侧)3组,建立实验性上颌窦炎模型,并采用窦口开放、扩大术治疗鼻窦炎。分别采用99mTc-SC核素法和活性碳粉末法观察上颌窦黏膜纤毛的运动。结果:99mTc-SC核素法可很好显示纤毛的运动方向;活性炭粉末测量法显示纤毛运动速度正常对照>鼻窦炎术后>鼻窦炎,三者间差异具有统计学意义(P<0.01)。结论:用活性碳检测上颌窦黏膜纤毛运动是一种有效判断纤毛功能的方法。术后再生的黏膜纤毛功能低下。

Objective.. To observe the change of cilia movement in rabbit sinusitis model before and after sinusitis surgery. Methods： Forty New Zealand White rabbits were divided into 3 groups： normal control （35 sides）, sinusitis group （50 sides）, and post-surgery sinusitis group （40 sides）. The experimental maxillary sinusitis model was made by bacteria inieetion and ostia closure. Rabbits with sinusitis were treated by opening and enlarging ostia of maxillary sinus. Activated charcoal powder and ^99mTc-SC methods were used to observe the mucociliary transport （MCT） in maxillary sinus. Results, ^99mTc-SC method satisfactorily reveal the direction of clia movement. Activated charcoal powder showed that the order of MCT velocity in 3 groups was： normal control group〉post-operation group〉pre-operation group （all P〈0.01）. Conclusion： Activated charcoal powder is an effective method in estimating the function of maxillary sinus cilia. The function of cilia of post-operative regenerated mucosa is lower than that of normal mucosa cilia.