蛋白质组技术在胃癌相关标志物筛查中的应用

Application of technologies of Proteome for screening molecular biomarkers associated with gastric cancer

目的建立胃癌组织和正常胃组织双向凝胶电泳图谱,鉴定差异表达蛋白,从中发现有意义的胃癌相关标志物。方法采用双向凝胶电泳(2-DE)分离胃癌组织和正常胃组织总蛋白,银染显色,PDQuest软件分析,从中选取差异表达蛋白质点,通过基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)或MALDI-TOF-TOF-MS鉴定差异表达的蛋白质。结果获得重复性和分辨率较好的胃组织双向凝胶电泳图谱；发现23个差异表达蛋白质,其中15个得到鉴定,在肿瘤组织中高表达的有10个,其余5个在肿瘤组织中低表达。结论建立了胃癌组织和正常胃组织双向凝胶电泳图谱,并应用质谱技术鉴定了15个差异表达蛋白质。

Objective To establish two-dimensional electrophoresis （2-DE） profiles of human gastric cancer tissue and paired normal tissue, and to identify differential expression proteins, in which try to find out candidates as gastric cancer-related proteins. Methods 2-DE was applied to separate the total proteins of gastric cancer tissue and paired normal tissue. After staining and analyzing by PDQuest software,the differential expression proteins were identified by matrix-assisted laser desorption/ionizationtime of flight mass spectrometry （MALDI-TOF-MS） or MALDI-TOF-TOF-MS. Results 2-DE profiles with high resolution and reproducibility were obtained. Twenty-three protein spots were found as differentially-expressed proteins, in which 15 protein spots were identified successfully. Among the identified proteins, there were 10 over-expressed and 5 under-expressed proteins in gastric cancer tissue as compared with normal tissue. Conclusion The well-resolved, reproducible 2-DE patterns of human gastric cancer tissue and paired normal tissue were established and fifteen differentially expressed proteins were identified.