槲皮素对TRAMP-C2前列腺癌细胞的抑制作用

Inhibitory effect of quercetin on angiogenesis in TRAMP-C2 prostate cancer cells in vivo

目的探讨槲皮素对TRAMP-C2前列腺癌小鼠模型的抑制作用及其机制。方法建立前列腺癌细胞株TRAMP-C2小鼠模型并随机分成4组。A组(对照组)；B组(槲皮素25 mg/ kg体重治疗组)；C组(槲皮素50 mg/kg体重治疗组)；D组(槲皮素100 mg/kg体重治疗组)。观察槲皮素对各组小鼠的抑瘤作用并检测各组肿瘤标本的微血管密度计数。结果接种5周后A、B、C、D各组小鼠的肿瘤重量分别为(1．85±0．23)、(1．61±0．10)、(1．10±0．17)、(0．79±0．11)g,治疗组明显低于对照组(P＜0．01),B、C、D组的抑瘤率分别为13%、41%、57%。各组肿瘤的微血管密度计数分别为39．29±6．39,31 61±4．82,23．42±3．91,14．00±4．01,治疗组明显减少(P＜0．05)。结论槲皮素可抑制前列腺癌细胞株TRAMP-C2小鼠移植瘤的生长；其机制可能与抑制肿瘤组织血管生成有关。

Objective To study the effect of quereetin on the growth of tumors established by TRAMP-C2 cells in mice and to explore the mechanisms of the effect. Methods TRAMP-C2 prostate cancer model was set up and the mice were randomly divided into four groups. Group A was used as control;25 mg/kg quereetin was given to each mouse in group B,while 50 mg/kg quereetin in group C and 100 mg/kg quereetin in group D. The inhibitory effect on tumors in each group was studied. Mierovessel density （MVD） was calculated by immunoehemieal methods. Results Quereetin inhibited the growth of mouse prostate cancer in vivo. The weight of tumors in the study groups was significant less than in the control group （P〈 0.01）. MVD in the study groups was decreased significantly （P〈 0.01）. The inhibitory effect was dose-dependent, and the inhibitory rate in groups B, C and D was 13 %, 41% and 57 % respectively. Conclusion The mechanism by which quereetin inhibits the growth of TRAMP-C2 prostate cancer cells in vivo might be related to the inhibition of tumor angiogenesis.