孔雀微卫星引物筛选及其遗传多样性分析

Screening of Peafowl Microsatellite Primers and Analysis of Genetic Diversity

利用29对鸡微卫星标记对孔雀基因组DNA进行种间扩增,发现14对引物能扩增出特异性条带,每对引物扩增的平均等位基因数为1.71,有7对引物具有较丰富的多态性,其中MCW0080和MCW0098最为理想。蓝孔雀和绿孔雀群体间和群体内的遗传分析结果表明,绿孔雀和蓝孔雀两个群体的期望杂合度分别为0.7422和0.6943,群体间的遗传分化系数为0.078,Reynolds’遗传距离和基因流分别为0.0603和3.896,结果显示这两个孔雀群体的杂合度和遗传多样性水平都很低,且有相互混杂的趋势。

The applicability of chicken microsatellite primers to peafowl population was analyzed in the present paper, and the results showed 14 of 29 pairs of microsatellite primers from chicken could amplify peafowl DNA and produce specific allele pattems. A mean of 1.71 alleles was found for each locus. Seven pairs were highly polymorphic, and MCW0080 and MCW0098 were ideal markers for peafowl. Genetic diversity analysis within and between the green peafowl and the blue peafowl populations demonstrated that the expected heterozygosity of two peafowl populations were 0.2482 and 0.2744, respectively. The inbreeding index （FST）, Reynolds＇ genetic distance and gene flow between the two populations were 0.078, 0.0603 and 3.896 respectively. These results indicate that the heterozygosity and the genetic diversity of these two peafowl populations were very low, and suggest a tendency towards intermixing.