尖锐湿疣及鲍恩样丘疹病皮损中高危型人乳头瘤病毒感染与端粒酶表达的关系

Relationship between high-risk HPV infection and expression of telomerase in condyloma acuminatum and bowenoid papulosis

目的:研究尖锐湿疣(CA)与鲍恩样丘疹病(BP)皮损中高危型人乳头瘤病毒(HPV)感染与端粒酶表达的关系。方法:采用PCR方法检测HPV类型,免疫组化方法检测CA和BP皮损中人类端粒酶反转录酶(hTERT)的表达。结果:CA和BP皮损中hTERT表达均比正常对照组高(P均<0.01),BP又较CA表达高(P<0.01)。CAHPV16阳性组hTERT表达较HPV16阴性组高,其差异有统计学意义(P<0.05);BP不同HPV型之间hTERT表达的结果与CA相同;以上各组均比正常对照组表达强度高(P<0.05)。结论:非恶性增殖性疾病中亦有端粒酶不同程度的表达;以HPV16为代表的高危型HPV感染与端粒酶表达有相关性。

Objective： To explore the relationship between high-risk HPV infection and expression of telomerase in condyloma acuminatum （CA） and bowenoid papulosis （BP）. Methods： HPV was typed by polymerase chain reaction （PCR）. The expression of human telomerase reverse transcriptase （hTERT） in the lesions was detected by immunohistochemistry technique. Results： In the expression of hTERT, both CA and BP were significantly higher than control, and the expression in BP was higher than that in CA. In CA, there was significant statistic difference in the expression of hTERT between HPV16-positive group and HPV16-negative group; the same result was also found in BP. In addition, the expression of hTERT in all the above groups was significantly higher than that in normal foreskin. Couelusions： The expression of telomerase can also be found in non-malignant hyperplastic diseases with slightly increase pattern. High-risk HPV infection is related to expression of telomerase and the causal relation ship between them needs further investigation.