脑衍生的神经营养因子及其受体trk B在老年性大鼠耳蜗中的表达

Expression of BDNF and trk B in the cochleas of the aged rats

目的:探讨脑衍生的神经营养因子(BDNF)及其受体trk B在老年性大鼠耳蜗中的表达。方法:采用实时定量PCR的方法对正常老年大鼠(老年非耳聋组)与老年性耳聋大鼠(老年耳聋组)及非老年正常大鼠(对照组)耳蜗组织中的BDNF和trk BmRNA表达进行定量分析。结果:定量分析结果显示,老年耳聋组耳蜗神经元和毛细胞中BDNF的表达比对照组明显减少(P<0.01);老年非耳聋组BDNF的表达与对照组比较,差异无统计学意义(P>0.05)。表达于神经元的trkB在老年耳聋组、老年非耳聋组和对照组差异均无统计学意义(P>0.05)。结论:老年性大鼠耳蜗中BDNF的表达与老年性大鼠耳聋的发病机制可能密切相关。

Objective： To explore the correlation between the expression of brain derived neurotrophic factor （BDNF） and presbycusis in the cochleae of the aged rats. Method：The mRNA expression of BDNF and presbycusis in the cochleas of the aged rats was quantitated by real time PCR. Result：The expression of BDNF in the cochleas of the aged rats with presbycusis was obviously less than that in the control group. And the difference was statistically significant. Conclusion：The expression of BDNF is closely correlated to presbycusis in the aged rats.