胰酶阻断经络的针刺效应实验

An experiment on pancreatic enzyme for blocking the meridian

目的:观察胰酶损伤心包经沿线脂肪条带结构后对针刺内关穴失血性休克家兔血压的影响。方法:实验于2003-03/2004-04在南通大学基础医学院机能学实验室完成。①选用健康新西兰家兔12只,雌雄不拘。按随机摸球法将家兔分为3组:对照组、胰酶损伤针刺组和针刺组,每组4只。麻醉家兔后,胰酶损伤针刺组:左前肢内侧皮下注射胰酶(Sigma公司产品,批号:1720856,5kU/瓶)0.4mL进行预处理,先分离气管并插入气管插管,再分离左侧颈总动脉且插入动脉插管,并采用由江苏生物医学工程学会医学电子技术研究所研制DOCTOR-95生物信号处理系统和复旦大学传感器工程研究室提供的PT14系列硅压力传感器测量血压,分离左侧股动脉插管准备放血。术后约30min左右待血压稳定后测定基础血压,然后缓慢放血,使颈动脉血压低于30mmHg(1mmHg=0.133kPa)持续15min。针刺组除不进行胰酶预处理外,其余干预方法同胰酶损伤针刺组。对照组只放血造成失血性休克,不另作处理。胰酶损伤针刺组和针刺组于家兔失血性休克后15min时在左侧内关处针刺15min(所用针型号GaugeNo.34,由中美合资苏州针灸器械有限公司生产),提捻交替进行。每只家兔均进行一次针刺。②于休克后45,60,75,90和105min分别记录各组家兔血压变化。③多组间计量资料差异比较采用单因素方差分析。结果:①对照组在休克120min时死亡2只,血压下降明显,150min时全部死亡。胰酶损伤针刺组在休克120min时死亡1只,且存活的3只血压均<30mmHg,150min后又死亡1只。针刺组在休克150min时无死亡,且血压均>40mmHg。②对照组家兔休克后45,60,75,90和105min颈动脉血压分别为(38.253±4.19),(36.75±5.06),(34.25±4.11),(31.25±5.06),(28.75±4.57)mmHg,与胰酶损伤针刺组相近[(42.00±4.32),(52.50±5.44),(49.25±3.86),(37.25±5.12),(34.25±3.30)mmHg],均明显低于针刺组[(54.50±8.42),(67.50±4.36),(68.50±4.20),(60.25±6.65),(51.25±2.36)mmHg,F=8.10～71.46,P<0.01]。结论:针刺内关穴可升高失血性休克家兔血压,胰酶对针刺失血性休克家兔“内关”心包经的行经传导有一定的阻断作用。

AIM： To study the blood pressure of rabbits with hemorrhagic shock induced by inner-pass acupuncture following the structure of adipo-strap along pericardium was damaged by pancreatic enzyme. METHODS： The experiment was performed in the Functional Laboratory of Basic Medical College of Nantong University from March 2003 to April 2004. ①Twelve healthy New Zealand male and female rabbits were selected and randomly divided into 3 group： the control group, the group of acupuncture after damaged by pancreatic enzyme （the injury group） and the acupuncture group with 4 rabbits in each group. Rabbits in the injury group were pretreated with subcutaneous injection of 0.4 mL of pancreatic enzyme （manufactured by Sigma Co., Ltd. with the batch number of 1720856, 5 kU/bottle） in the left anterior limb. The trachea was separated and inserted with a trachea cannula, then the left common carotid artery （CCA） was separated and inserted with an arterial cannula to record the blood pressure by the DOCTOR-95 bio-signal processing system manufactured by the Laboratory of Electronic Technique of Jiangsu Provincial Institute of Medical Engineering and the PT14 pressuresensitive element made by the Laboratory of Sensor Engineering of Fudan University. The left femoral artery was separated and inserted with an arterial cannula for blood-letting. The basic blood pressure was measured at 30 minutes after operation when the blood pressure was stable. The blood was let out slowly to keep the CCA blood pressure below 30 mmHg for 15 minutes. Rabbits in the acupunctural group were treated the same as the injury group without injection of pancreatic enzyme. Rabbits in the control group were induced into hemorrhagic shock by blood letting without any other treatments. At the 15＇h minute after hemorrhagic shock, rabbits in both of the injury group and the acupunctural group were needled at the left inner pass for 15 minutes （needles of Gauge No.34 were adopted, which were manufactured by China-American Suzhou Acupuncture Equipment Co., Ltd.）, and the needling was lift and punctured in turn, and each rabbit was needled only once ② The changes in blood pressure were recorded in rabbits of each group respectively at the 45^th, 60^th, 75^th, 90^th and 105^th minutes after shock. ③ Differences in measurement data among groups were compared with single factor analysis. RESULTS： ① Two rabbits in the control group died in the 120^th minute after shock with blood pressure decreased obviously, and all of the rabbits died in the 150^th minute. One rabbit in the injury group died in the 120^th minute after shock, and the blood pressure of the other three rabbits were below 30 mm Hg, 150 minutes later another rabbit died. There was no rabbit died in the acupunctural group in the 150^th minute, whose blood pressure were greater than 40 mm Hg. ② The blood pressures of carotid artery in rabbits of the control group were （38.253 ±4.19）, （36.75 ±5.06）, （34.25 ±4.11）, （31.25 ±5.06）, （28.75 ±4.57 ） mm Hg respectively at the 45^th, 60^th, 75^th, 90^th and 105^th minutes, which were similar to those in the injury group [（42.00±4.32）, （52.50±5.44）, （49.25±3.86）, （37.25±5.12）, （34.25±3.30） mm Hg], and they were obviously .lower than those in the acupunctural group [ （ 54.50 ±8.42 ）, （67.50 ±4.36 ）, （68.50 ±4.20 ）, （ 60.25 ±6.65 ）, （ 51.25 ±2.36） mm Hg,F=8.10-71.46, P 〈 0.01]. CONCLUSION： Acupuncture of the inner pass can elevate the blood pressure of rabbits with hemorrhagic shock, and the pancreatic enzyme has blockage effect to some extent on the conduction of menstruate by acupuncture the inner pass pericardium meridian in rabbits with hemorrhagic shock.