低氧暴露对运动诱导的血红蛋白低下大鼠红细胞参数和某些造血因子的影响

Effects of Intermittent Hypoxic Exposure on the Production of Erythrocyte and Some Haematopoietic Factor in Rats with Exercise-Induced Anemia

目的:探讨低氧暴露对运动性血红蛋白低下机体红细胞参数和某些造血因子的影响及其机制。方法:采用6周递增负荷跑台运动建立大鼠运动性血红蛋白低下模型,人工常压低氧环境下(14.5%O2)分别采用每天1h、2h和(1+1)h三种暴露方式进行恢复,3周后测定运动性血红蛋白低下大鼠血红蛋白含量(Hb)、红细胞数目(RBC)、红细胞压积(Hct)、肾脏促红细胞生成素(EPO)与血清白细胞介素-3(IL-3)含量,并与常氧恢复组比较。结果:1h、2h和(1+1)h低氧暴露恢复组大鼠的Hb、RBC、Hct水平均显著高于常氧恢复组(P<0.05,P<0.01),肾脏EPO水平显著高于常氧恢复组(P<0.05),而血清IL-3水平虽有增高趋势,但无显著意义(P>0.05)。三种低氧暴露方式大鼠各指标之间未见显著性差异(P>0.05)。结论:低氧暴露能加速红细胞的生成,有助于运动性血红蛋白低下的恢复,其作用机理可能与低氧暴露提高机体某些红细胞造血生长因子水平有关。

Objective To explore the effects of intermittent hypoxic exposure on the parameter of erythrocyte and some haematopoietic factors in rats with exercise - induced anemia, and study the mechanism of preventing and treating exercise - induced anemia through hypoxic exposure. Methods A rat model of exercise - induced anemia was established through 6 - week treadmill training with progressive loading. With the model, erythrocyte parameters, kidney EPO and serum IL - 3 levels were investigated under hypoxic exposure （14.5% of oxygen） for 1 hour, 2 hours and intermittent exposure （1 ＋ 1 hour） Results After hypoxic exposure, the hemoglobin （Hb）, red blood cell （RBC） and haematocrit （Hct） levels increased significantly （P 〈 0.05 and P 〈 0.01, respectively）. The kidney EPO levels increased significantly （P 〈 0.05）. Serum IL-3 level revealed no significant difference （ P 〉 0.05）, though with an augmented tendency. The three exposures ways showed no significant difference in the above parameters （ P 〉 0. 05）. Conclusion The hypoxic exposure can accelerate the production of erythrocyte, and treat the exercise - induced anemia effectively. Improvement of some haematopoietic factors and enhancement of hematopoiesis in marrow is thought to be the possible mechanism.