黏膜相关淋巴组织结外边缘区B细胞淋巴瘤BCL-10蛋白表达及其与API2-MALT1融合基因的关系

Expression of BCL-10 protein and the relationship with API2-MALT1 fusion gene in extranodal marginal zone B-cell lymphoma of mucosa associated lymphoid tissue

目的探讨MALT淋巴瘤中BCL-10蛋白核表达与AP12-MALT1融合基因的关系。方法对86例MALT淋巴瘤进行回顾性研究,采用SP法检测BCL-10的表达,RT-PCR检测AP12- MALT1融合基因。结果10例桥本甲状腺炎均表达BCL-10于胞浆,86例MALT淋巴瘤患者中,42例(48．8％)同时表达BCL-10于胞核和胞浆,35例(40．7％)检测出AP12-MALT1融合基因。BCL-10核表达的检出与AP12-MALT1融合基因具有相关性(r=0．374,P=0．000)。结论MALT淋巴瘤中BCL-10蛋白的核表达与AP12-MALT1融合基因相关。

Objective To investigate the relationship of BCL-10 protein and API2-MALT1 fusion gene in MALT lymphoma. Methods Specimens from 86 cases of MALT lymphoma were studied by immunohistochemical staining for BCL-10. RT-PCR was used to detect the transcripts of API2-MALT1 fusion gene. Results In all 10 cases of Hashimoto thyroiditis only cytoplasmic BCL-10 expression in lymphoid cells was observed. In 86 MALT lymphoma cases, 42 cases （48.8%） exhibited BCL-10 expression in both nucleus and cytoplasm. API2-MALT1 fusion gene was detected in 35 cases （40.7%） of MALT lymphoma. BCL-IO nuclear expression was correlated with API2-MALT1 fusion gene transcript （ r = 0. 374,P = 0. 000）. Conclusion BCL-10 nuclear expression is correlated with API2-MALT1 fusion gene expression in MALT lymohoma.