压应力下兔软骨细胞的凋亡和IL-1β、TNF-α的相关性研究

Effects of chondrocyte apoptosis and IL-1β、TNF-α under compressive stress

目的:观察持续及间歇压应力下关节软骨的组织学变化、软骨细胞的凋亡并测定关节液中细胞因子(IL-1β、TNF-α),探讨压应力下软骨细胞凋亡和关节软骨退变的相互关系及压应力下软骨细胞凋亡的相关机制。方法:在活体新西兰大白兔左后肢的髌股关节间造成持续及间歇的不同压应力模型,60只新西兰大白兔,随机分为7组:A组,对照组;B组,持续伸直位;C组,持续屈曲80°位;D组,持续屈曲140°位;B1组,间歇伸直位;C1,间歇屈曲80°位;D1组,间歇屈曲140°位。持续组于1周、2周、4周、6周、8周,间歇组于4周、8周、12周先进行髌股关节间压应力测试,然后取材利用HE染色、甲苯胺蓝染色、TUNEL法等方法观察关节软骨细胞及关节软骨的组织学变化,并采用酶联免疫检测方法测定关节液中细胞因子的含量。结果:关节间持续及间歇的较高压应力可以引起关节软骨细胞凋亡并伴有关节软骨退变,并同时伴有关节液中细胞因子含量的升高;持续的压应力引起的变化大于间歇的压应力;较长时间的关节制动也可以引起上述变化。结论:关节间持续及间歇的较高压应力可以引起软骨细胞凋亡,并继而出现软骨退变,IL-1β和TNF-α在软骨细胞凋亡过程中可能起重要作用。

Objective：To investigate the histologic changes of articular cartilage and the apoptosis of chondrocyte under continuous or intermittent compressive stress. To explore the relationship between chondrocyte apoptosis and the degeneration of cartilage,the possible mechanism of chondrocyte apoptosis under compressive stress. Method： Different continuous and intermittent compressive press model were designed between the patella and femur. 58 New Zealand white rabbits were randomly grouped into 7：group A：control group： group B：continuous straight; group C： continuous bend 80°; group D：continuous bend 140°;B1 group：intermittent straight;C1 group：intermittent bend 80°;D1 group：intermittent bend 140°. Continuous groups were killed at the time of lweek,2week,4week, 8week,intermittent groups were killed at the time of 4week,8week,12week, Before killing,the compressive press between the patella and femur was firstly measured.The histologic changes of cartilage were investigated. The apoptosis of chondrocyte was detected. The amount of cytokines was analysed through ELISA method. Result：The higher compressive stress can induce the apoptosis of chondrocyte,then lead to the degeneration of cartilage. The amount of cytokine in synovial fluid also increased at the same time, The changes under the continuous compressive stress were more. The long period immobilization could induce the same changes, Conclusion：The higher compressive stress can induce the apoptosis of chondrocyte and lead to the degeneration of cartilage. IL-1β and TNF-α may play an important role in the apoptosis of chondrocyte.