抑癌基因PTEN、癌基因MDM2在肾透明细胞癌中的表达及意义

Expression of PTEN and MDM2 protein in renal clear cell carcinoma

目的:探讨抑癌基因PTEN蛋白、癌基因MDM2蛋白在肾透明细胞癌发生中的意义及其对肾透明细胞癌患者预后的影响。方法:采用免疫组织化学SABC法检测58例肾透明细胞癌、15例癌旁及10例正常肾组织中抑癌基因PTEN、癌基因MDM2蛋白的表达。结果:58例肾透明细胞癌组织中PTEN阳性表达率为34.4%(20/58)显著低于癌旁肾组织86.6%(13/15)及正常肾组织90%(9/10),差异有统计学意义(P<0.01),癌旁肾组织和正常肾组织阳性表达率之间差异无统计学意义(P>0.05);Ⅰ、Ⅱ期肾透明细胞癌组织阳性表达率72.7%(16/22)显著高于Ⅲ、Ⅳ期的11.1%(4/36),差异有统计学意义(P<0.01)。58例肾透明细胞癌组织中MDM2阳性表达率为68.9%(40/58)显著高于癌旁肾组织0.06%(1/15)及正常肾组织的0(0/10),差异有统计学意义(P<0.01),癌旁肾组织和正常肾组织阳性表达率之间差异无统计学意义(P>0.05);Ⅰ、Ⅱ期肾透明细胞癌组织阳性表达率为45%(10/22)显著低于Ⅲ、Ⅳ期的83.3%(30/36),差异有统计学意义(P<0.01)。PTEN和MDM2二者高度相关。结论:PTEN的失表达与MDM2的过表达在肾透明细胞癌的发生中起一定的作用,且与肾透明细胞癌的发展和淋巴结转移密切相关,PTEN与MDM2可作为评价肾透明细胞癌发展和预后的生物学指标。

Objective： To investigate the expression of PTEN and MDM2 in the pathogenesis and prognosis of renal clear cell carcinoma. Methods： The expression of PTEN and MDM2 protein were evaluated in 58 renal clear cell carcinoma tissues, 15 renal tissues adjacent to carcinoma tissues and 10 normal renal tissues by immunohistochemistry method （SABC method）. Results： PTEN was expressed in 34.4% （20/58）of in all the renal clear cell carcinoma patients, which was statistically significant lower than that in renal tissues adjacent to carcinoma tissues （86.6%, 13/15） and in the normal renal tissues （90%, 9/10） （both P 〈 0.01）, while it was not markedly different in renal tissues adjacent to carcinoma tissues and the normal renal tissues（ P 〉 0.05）. PTEN expressed in Stage Ⅰ and Ⅱ renal carcinoma tissues （72.7%, 16/22） was much higher than that in Stage Ⅲ and Ⅳ carcinoma tissues （11.1%, 4/36）. MDM2 was expressed in 68.9% （40/58）of all the renal clear cell carcinoma patients, which was statistically significant higher than that in renal tissues adjacent to carcinoma tissues （0.06%, 1/15） and the normal renal tissues （0, 0/10） （both P 〈 0.01 ）, while it was not significantly different in renal tissues adjacent to carcinoma tissues and the normal renal tissues （P 〉 0.05）, MDM2 expressed in Stage Ⅰ and Ⅱ renal carcinoma tissue （45%, 10/22） was markedly lower than that in Stage Ⅲ and Ⅳ carcinoma tissue （83.3%, 30/36）. The expression of PTEN was highly related with that of MDM2. Conclusions： The deletion or weak expression of PTEN and over-expression of MDM2 play a eertain role in the oeeurrenee of primary renal elear cell eareinoma and closely correlate with the development of renal eareinoma and lymphatie metastasis, which indicates that PTEN and MDM2 can be valued as biological indexes for development and prognosis of primary renal clear cell eareinoma.