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NSAIDs对结肠癌细胞生长的抑制作用及对VEGF表达的影响

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摘要 目的明确非甾体抗炎药(NSAIDs)对体外培养结肠癌细胞SW1116生长的影响及血管内皮生长因子(VEGF)表达的调控。方法采用MTT法观察NSAIDs对SW1116细胞生长的影响;电镜下观察尼美舒利诱导细胞凋亡的形态学变化;ELISA法检测细胞上清液中VEGF的含量。结果NSAIDs能呈剂量和时间依赖性抑制结肠癌SW1116细胞生长;加入尼美舒利后,上清液中VEGF的含量随作用时间的延长逐渐下降,作用72h后电镜下见凋亡小体。结论NSAIDs可明显抑制SW1116细胞生长,尼美舒利可诱导其凋亡,下调VEGF的表达,从而抑制结肠癌新生血管的形成。
作者 费素娟 陈光侠 季雪静 刘军权 陈复兴
机构地区 徐州医学院附属医院 中国人民解放军第九七医院
出处 《江苏医药》 CAS CSCD 北大核心 2006年第10期970-971,共2页 Jiangsu Medical Journal
基金 江苏省普通高校自然科学研究计划资助项目(01KJB320011)
关键词 结肠癌细胞 非甾体抗炎药 血管内皮生长因子
分类号 R735.35 [医药卫生—肿瘤]
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参考文献9

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江苏医药
2006年 第10期

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