葡多酚对肝细胞内Ca^(2+)浓度和增殖活性的影响

Effects of grape procyanidins on the concentration of intracellular calcium and the proliferation activity of the hepatoma cells

目的探讨葡多酚(GPC)对正常肝细胞和受乙醇损伤肝细胞内Ca2+浓度与细胞增殖活性的影响。方法将大鼠正常肝细胞和乙醇损伤肝细胞加入不同剂量GPC共同培养,用Fura-2荧光测定肝细胞内Ca2+浓度,用MTT法测定细胞增殖活性。结果①正常对照组和乙醇对照组的Ca2+浓度分别为(108·26±14·17)和(651·24±47·95)nmol/L,二者差异有显著性意义(P<0·05);中、高剂量GPC组均较乙醇对照组显著性降低(P<0·05)。②加入GPC的正常肝细胞内Ca2+浓度依次为:细胞外液含Ca2+组>外液无Ca2+组>正常对照组。③正常肝细胞和乙醇损伤肝细胞的中、高剂量GPC组细胞增殖活性均较正常对照组和乙醇对照组显著性升高(P<0·05)。结论GPC可通过增加细胞外液Ca2+内流和胞内Ca2+库的释放两种途径升高正常肝细胞内Ca2+浓度,提高细胞增殖活性,并可抑制乙醇引发的肝细胞内Ca2+浓度异常升高(超载)和细胞增殖活性损伤。

Objective To investigate the effects of grape procyanidins（GPC） on concentration of intracellular calcium and the proliferation activity of normal hepatic cells and the hepatic cell injuried by alcohol. Methods Rat hepatic cells and the cell injuried by alcohol were cultured with different concentration of GPC. The proliferation activity and concentration of intracellular calcium of the hepatic cells were measured by MTT assay and Fura-2 fluorescence methods. Results ①The concentration of intracellular calcium of the normal control group and alcohol injury group were （108.26±14.17） and （651.24± 47.95）nmol/L respectively, and that of both the high and the medium dose GPC groups were lower than the alcohol injury group,all differences are significant（ P 〈 0.05）.②The concentration of intracellular calcium in the normal hepatic cells treated with GPC is the group with calcium of extracellular fluid 〉 the group free from calcium of extracellular fluid 〉 the normal control group.③ The proliferation activity of the high and the medium dose GPC group of normal hepatic cell and the cell injuried by alcohol were higher than the normal control group and alcohol injury group, all differences are significant （ P 〈 0.05）. Conclusion GPC can raise the intracellular calcium concentration of normal hepatic cell by increasing extracellular fluidca introaffluxion and release from calcium pool, and enhance the proliferation activity of hepatic cells. It also can inhibit the abnormal rise （overload） of intracellular calcium concentration and the proliferation activity injury of hepatic cell induced by alcohol.