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丙型肝炎病毒非结构蛋白NS4B抑制IFN介导的抗病毒反应 被引量：2

Inhibition of IFN-γ receptor signaling by Hepatitis C Virus non-structural protein NS4B
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摘要 丙型肝炎病毒(Hepatitis C Virus,HCV)非结构蛋白NS4B的功能仍不很清楚。本实验旨在研究NS4B对干扰素介导的抗病毒反应的影响。建立稳定表达NS4B的细胞系后,用空斑实验研究NS4B在不同浓度的IFN_α下对水泡口炎病毒(VSV)的影响,利用代表2308个信号传导相关的基因的微点阵研究其对细胞基因表达的影响,和流式细胞仪分析IFNGR1的荧光强度。结果显示HCV_NS4B能微弱地抑制IFN_α介导的抗病毒反应,可能原因是HCV_NS4B抑制一些与免疫反应相关的基因的表达,特别是与IFN_γ信号传导有关的基因。因此,NS4B对HCV耐受干扰素治疗起一定的作用。 The function of NS4B is incompletely understood. The aim of the study is to understand the influence of NS4B on anti-viral response. After cell line stably expressing NS4B established, the influence of IFN-αof different concentration on VSV was studied using plaque assay; cell expression profiling caused by NS4B was studied using DNA microarray, and the IFNGR1 fluorescence intensity was analyzed. Our data showed that HCV-NS4B could suppress immunoassociated gene expression, in particular, IFN-γreceptor signal transduction-related genes. Taken together, NS4B could play some roles in HCV resistance to IFN therapy.
作者 郑义 罗海波 高博 黄来强
机构地区 清华大学深圳研究生院生命科学部 武汉大学生命科学学院病毒学国家重点实验室
出处 《微生物学报》 CAS CSCD 北大核心 2006年第5期802-806,i0001,共6页 Acta Microbiologica Sinica
关键词 丙型肝炎病毒 非结构蛋白4B 空斑实验 DNA微点阵 Hepatitis C Virus Non-structural protein 4B DNA microarray
分类号 R373 [医药卫生—病原生物学]
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参考文献21

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微生物学报
2006年 第5期

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