阳极氧化铝膜过滤BSA溶液过程中的污染分析

Fouling of bovine serum albumin on the anodic aluminum oxide membrane

采用阳极氧化铝膜过滤牛血清蛋白溶液,考察了孔径20、100、200 nm 3种膜的过滤通量随时间的变化关系,在相同的操作条件下孔径20 nm的膜具有较高的渗透通量(400 L/(m2.h))和较小的过滤阻力(8.3×1011/m)。还考察了蛋白质浓度、泵送时间及清洗对膜污染情况的影响。结果表明,在实验的范围和条件下,随蛋白质浓度的增大,膜污染加重,稳定通量增大;泵送时间增长,膜污染速度加快,通量增大;通过纯水清洗加超声清洗的方法去除可逆污染,清洗后膜与新膜过滤通量相当。

Flux decline of aqueous solution of Bovine Serum Albumin （BSA） was studied when it was tangentially filtered through three anodic aluminum oxide membranes with nominal pore sizes of 200 nm, 100 nm and 20 nm made by Whatman under a constant pressure of 0. 1 MPa. The membrane with pore size of 20 nm had a higher steady flux and lower filtration resistance. Concentrations of BSA influenced the flux sharply. It would get higher steady flux in the system with higher concentration, because the fouling with gel layer could reach steady faster . The flux was also affected by pumping time, and the membrane flux was higher with longer pumping time.