黄瓜子叶节再生体系优化研究

Optimum study of in vitro culture and plantlet regeneration system from cotyledonary nodes of cucumber(Cucumis sativus L.)

研究了TDZ和IBA浓度、苗态、外植体切割和接种方式及基因型对黄瓜子叶节再生体系建立的影响。结果表明,最佳芽诱导培养基为M S+0.005 m g/L TDZ+0.05 m g/L IBA;两片子叶脱离种壳且与下胚轴处于弯曲状态的子叶节再生频率最高;保留单片子叶、下胚轴纵切、切除此子叶的2/3且竖直插入为最佳切割和接种方式;农城3号为最佳基因型,每块外植体再生芽数可达1.89。

The main factors influencing the plantlet regeneration system from cotyledonary nodes of cucumber （Cucurnis sativus L. ） were discussed. The results showed that concentration of TDZ and IBA,stage of cotyledonary nodes,types of cutting and implantation of explants,and genotypes had effect on establishing the regeneration system of cucumber. The optimum culture medium to induce buds was the medium of MS＋0. 005 mg/L TDZ＋0.05 mg/L IBA. Stage three of the cotyledonary nodes ,which had two cotyledons separating from its husk and were in bent status with its hypocotyl,gave the maximum number of buds per explant. The best way of cutting was to cut hypocotyl longitudinally cut the cotyledon by 2/3 and insert it vertically. Nongcheng No. 3 was the optimum genotype and the maximum number of buds was 1.89 per explant.