摘要
目的:研制复方胰岛素凝胶并建立其含量测定方法。方法:以卡波姆为基质制备复方胰岛素凝胶;采用紫外分光光度法测定胰岛素含量,并考察其稳定性及对家兔皮肤的刺激性。结果:胰岛素检测浓度在10~150μg/ml范围内线性关系良好,线性方程为A=8.75120×10^-4C-0.00245(r=0.99976,n=3),平均加样回收率为100.46%,RSD=1.26%;所得凝胶在室温或4℃冰箱中存放稳定性好,对家兔皮肤未见刺激性发生。结论:该制剂制备工艺简单,稳定性好,检测方法快速、准确。
OBJECTIVE: To prepare compound insulin gel and to establish a method for its content determination. METHODS:With carbomer as veihele,the compound insulin gel was prepared;An ultraviolet spectrophotometry for the content determination of insulin in gel was established,and the stability and irritation on rabbit of compound insulin gel was observed. RESULTS:The calibration curve of insulin was linear in the concentration range of 10- 150μg/ml, A = 8.75 120 × 10^-4C--0.00 245 (r = 0.99 976, n = 3) .The average recovery rate was 100.46%, RSD = 1.26% ; The gel was good stability under room temperature and 4℃environment, no sensitiveness on rabbit was observed . CONCLUSIONS : The technic of preparation was simple,and its stability was better,the method of content measured was rapid and accurate.
出处
《中国药房》
CAS
CSCD
北大核心
2006年第19期1474-1475,共2页
China Pharmacy
基金
广东省科技计划项目(2001C30801)
关键词
胰岛素
凝胶
研制
含量测定
Insulin
Gel
Preparation
Content determination