反相离子对高效液相色谱法测定枳实中辛弗林的含量

Content Determination of Synephrine in Citrus Auarntium L. by Reversed Phase Ion-pair HPLC

目的建立以反相离子对高效液相色谱(HPLC)法测定枳实药材中辛弗林含量的方法。方法色谱柱为HypersilODS2,流动相为乙腈-水-磷酸(32∶68∶0.2),检测波长为224nm,流速为1.0ml/min,柱温为30℃。结果辛弗林进样量在0.112μg^2.24μg范围内线性关系良好(r=0.9997),加样回收率为99.85%(RSD=0.96%,n=6)。结论本方法简便、可靠、无杂质干扰,可用于枳实药材的的质量控制。

OBJECTIVE： To establish a reversed phase ion pair HPLC method for the content determination of synephrine in Citrus aurantium L.. METHODS： The analysis was performed on hypersil ODS2, the mobile phase was CHiN H2O- H3PO4（32 ： 68 ： 0.2） with a flow rate of 1.0ml/min, the column temperature was 30℃ . RESULTS： There was a good linear relationship of synephrine sample size in the range of 0. 112μg -- 2.24μg （r ： 0.9 997）, and the recovery wa,s 99.85% （RSD= 0.96%, n = 6） . CONCLUSIONS： This method is simple and reliable without interference of impurity, which can be used for the quality control of Citrus aurantium L..