摘要
目的研究上皮性卵巢癌组织中P-糖蛋白、Survivin蛋白和拓扑异构酶Ⅱ的表达及其临床意义。方法采用免疫组化法检测34例卵巢上皮性癌、25例卵巢良性上皮性肿瘤和20例正常卵巢组织P-糖蛋白、Survivin蛋白和拓扑异构酶Ⅱ的表达。结果卵巢癌组织中P-糖蛋白、Survivin蛋白和拓扑异构酶Ⅱ的阳性表达率分别为29.41%(10/34)、64.71%(22/34)及50.00%(17/34),正常卵巢组织及卵巢良性上皮性肿瘤中P-糖蛋白、Survivin蛋白均未表达,拓扑异构酶Ⅱ均为阳性表达。卵巢癌组织中P-糖蛋白阳性表达率在各组织类型之间差异极显著(P<0.01),其中卵巢黏液性囊腺癌阳性表达率最高为66.67%(4/6);Survivin蛋白、拓扑异构酶Ⅱ的阳性表达率在各组织类型间差异无显著性(P>0.05);P-糖蛋白、Survivin蛋白、拓扑异构酶Ⅱ在卵巢上皮性癌的表达与病理分级及临床分期均无明显相关性(P>0.05)。结论联合检测P-糖蛋白、Survivin蛋白和拓扑异构酶Ⅱ的阳性表达率对卵巢癌化疗药物的选择具有指导意义,对预后判断有重要参考价值。
Objective To explore the expression of P - Glyeoprotein, survivin protein and topoisomerase Ⅱ （Topo Ⅱ ）in ovarian epithelial cancer tissues and its clinical significance. Methods Immunohistoehemistry was used for determination of the expression of P - gp, survivin protein and topo Ⅱ in 34 ovarian epithelial carcinoma eases, 25 epithelial benign ovarian tumors eases and 20 normal ovarian tissue eases. Results The positive expression rates of P - gp.survivin protein and topo Ⅱ in ovarian epithelial cancer tissues were 29.41% （10/34） ,64.71% （ 22/34 ） and 50.00% （ 17/34 ）, respectively. P - gp and survivin protein were negtive in normal ovarian tissues eases and epithelial benign ovarian tumors eases, while topo Ⅱ was positive in these two tissues. There was a significant difference among the three histological types of P - gp expression rate in ovarian epithelial cancer tissues （ P 〈 0.01 ）, that of mucinous cystodenocarcinoma was the highest,was 66.67% （4/6） .There was no sgnificnt difference in expression of survivin protein and topo Ⅱ in different histological types （ P 〉 0.05）. There were non significant difference in expression rates of P - gp, survivin protein and topo Ⅱ in epithelial ovarian cancer tissues at various tumor pathological grade and clinical stage （P 〉 0.05）. Conclusion Joint determination of P - gp, survivin protein and topo Ⅱ is valuable for selecting drugs and the estimation of the consequence.
出处
《中国热带医学》
CAS
2006年第10期1760-1761,共2页
China Tropical Medicine
