苦艾对伯氏疟原虫的抗疟实验研究

Experimental study on the effect of wormwood on mice infected with Plasmdium beighei

目的观察苦艾对感染伯氏疟原虫小鼠的疗效。方法按“四天抑制法”的d0感染,d0～d3给药,每天1次,d4涂薄血膜检查,并计算每天存活率及d4减虫率。结果不同浓度苦艾组疟鼠累积生存率与对照组比较,0.30g/ml组与0.45g/ml组差异有统计学意义(P<0.05);苦艾(0.30g/ml和0.45g/ml)组的d4减虫率与对照组相比差异有显著性;苦艾(0.15g/ml)组的d4减虫率与对照组相比差异无显著性。结论苦艾(0.30g/ml与0.45g/ml)能较好抑制伯氏疟原虫的生长,对小鼠的存活状况有改善作用。

Objective To investigate the therapeutic effect of wormwood in mice infected with Plasmodium berghei. Methods Groups of mice infected with P. Berghei were treated with oral wormwood of three concentrations （0.15g/ml, 0.30g/ml and 0. 45g/ml） daily based on＂4 - day suppress assay＇for a period of four days. Thin blood smears were made and examined after stained with Giemsa stain. The daily survival rate of mice and parasite reduction rate four days after treatment were observed. Results Compared with control group, the accumulated survival rate of mice in wormwood group（0.30g/ml and 0.45g/ml）was significantly different （P 〈 0.05）; Wormwood with the concentrations of 0.30g/ml and 0.45g/ml was significantly effective than that of wormwood with the concentration of 0.15g/ml. There no significant difference in anti - malaria effect was observed in wormwood group with the concentration of 0.15g/ml as compared with that of the control. Conclusion Wormwood with the concentration of 0.30g/ml and 0.45g/ml can inhibit the growth of malaria parasites and enhance the survival of mice infected with P. Berghei.