检测EB病毒TK激酶抗体方法的建立及其在鼻咽癌诊断中的应用

Establishment of Method for Detection of the Antibody to EBV-TK Kinase and its Application on the Early Diagnosis of Nasopharygeal Carcinoma

目的:建立检测鼻咽癌(NPC)患者血清中EB病毒TK激酶的酶免疫测定(EIA)方法,用于筛选和辅助诊断早期NPC患者。方法:用基因工程高效表达的EB病毒TK激酶作为包被抗原,建立EIA检测方法,检测NPC患者和对照血清中的TK/IgG抗体。结果:在NPC患者血清中检出了特异的对EB病毒TK激酶的IgG抗体,而正常人血清中为抗体阴性。结论:首先建立了简便、快速、特异和敏感的早期诊断NPC的EIA方法,并作为我公司产品扩展应用。

Objective： To establish an enzyme immunoassay（EIA） method for detection the Epstein-Barr virus（EBV）-TK/IgG antibody in sera of nasepharygeal carcinoma（NPC） patients and screening early NPC patients. Methods： The purified genetic engineered TK kinase was used in EIA to detect the IgG antibody in sera from NPC patients and other donors. Results： Specific IgG antibody against TK kinase was found in the sera of NPC patients. The positive rate of TK/IgG antibody in NPC patients, normal individuals and patients with tumor other than NPC was 97.8%, 0.16% and 0 respectively. The positive rate was 100% in Ⅰ and Ⅱ stage of NPC patients. Conclusion： The EBV TK kinase shows highly specificity and sensitivity in EIA. The results may be recommended as a simple specific and sensitive method for early diagnosis of NPC.