人血浆蛋白连续沉淀分离技术试验

Experiment for Continuous Fractionation of Human Plasma Proteins by Precipitation

目的:考察人血浆蛋白连续流沉淀分离装置的分离性能。方法:将传统的人血浆蛋白低温乙醇批处理法改进为连续沉淀分离法,并设计了一套连续沉淀分离装置。采用人血浆低温乙醇分离的组分FⅠ+Ⅱ+Ⅲ沉淀为试验物料,将蛋白液与试剂(乙醇和缓冲液)在特制的混合器内与循环母液迅速混合,形成连续沉淀分离过程。结果:实现了人血浆蛋白的连续沉淀分离,所得蛋白组分FⅡ中的IgG含量为5.0～8.4g/L,纯度达94%～97.4%。结论:该装置能够实现人血浆蛋白的连续分离,须进一步的试验和工艺优化。

Objective： To investigate the separation performance about the equipment with continuous fractionation of human plasma proteins. Methods： The conventional cold-ethanol batch fractionation method of human plasma proteins was converted to the continuous fractionation process. Designed a continuous fractionation equipment, F Ⅱ was precipitated from the precipitate of Cohn＇s factors Ⅰ＋Ⅱ＋Ⅲ by mixing proteins suspension, cold-ethanol and buffer liquid in the recycled product stream of the suspension. Results： Compared to the batch process, the continuous precipitation process had less time, and the contents of IgG in F Ⅱ were 5.0～8.4 g/L, and the purity of IgG in F Ⅱ was 94%～97.4%. Conclusion： The device can be used for fractionation of human plasma proteins, and make more experiments for optimal fractionation technics.