灵龟八法对肾阳虚模型豚鼠自由基代谢的影响

Effects of eight methods of intelligent turtle on free radical metabolism in the kidney-yang deficiency model guinea pigs

目的观察运用灵龟八法按时开穴灸对衰老的影响和作用机理。方法以健康成年豚鼠为研究对象,随机分为5组,肾阳虚模型灵龟八法预防组(Ⅰ组)、肾阳虚模型命门灸预防组(Ⅱ组)、肾阳虚模型灵龟八法治疗组(Ⅲ组)、肾阳虚模型命门灸治疗组(Ⅳ组)、肾阳虚模型对照组(Ⅴ组),以SOD、MDA为客观指标,比较灵龟八法按时开穴灸、命门灸对肾阳虚模型豚鼠自由基代谢的影响差异。结果在造模结束后,Ⅰ组及Ⅱ组没有出现肾阳虚征象,SOD、MDA的改变不明显;Ⅲ组、Ⅳ组及Ⅴ组均出现明显的肾阳虚症状,且SOD降低(P<0.01)、MDA升高(P<0.01或P<0.05);经治疗后,Ⅲ组的肾阳虚症状基本消失,SOD升高(P<0.01)、MDA降低(P<0.01),与造模前相比无明显差异(P>0.05);Ⅳ组的肾阳虚症状改善明显,SOD升高(P<0.05),但与造模前相比仍有差异(P<0.05),MDA降低(P<0.05),与造模前相比无显著性差异(P>0.05);而Ⅴ组的肾阳虚症状、SOD、MDA均无明显变化(P>0.05)。结论灵龟八法按时开穴灸、命门灸均可以有效调节肾阳虚模型豚鼠的自由基代谢,对肾阳虚模型豚鼠具有明显的预防及治疗作用,其抗衰老机理与调节自由基代谢密切相关;灵龟八法按时开穴灸对肾阳虚模型豚鼠的治疗作用略优于命门灸。

Objective To observe effects of moxibustion according to opening points on time in eight methods of intelligent turtle on aging and the mechanism. Methods Healthy and adult guinea pigs were randomly divided into 5 groups, a prevention group of kidney-yang deficiency model with eight method of intelligent turtle （group Ⅰ ）, a prevention group of kidney-yang deficiency model （group Ⅱ ） with moxibustion at Mingmen （GV 4）, a group of treatment group of kidney-yang deficiency model with eight methods of intelligent turtle （group Ⅲ ）, a treatment group of kidney-yang deficiency model （group Ⅳ ） with moxibustion at Mingmen （GV 4） and a control group of kidney-yang deficiency, Effects of moxibustion according to opening on time in eight methods of intelligent turtle and moxibustion at Mingmen （GV 4） on superoxide dismulase （SOD） activity and malondialdehyde （MDA） level were compared, Results After modeling, no signs of kidney-yang deficiency and no significant changes of SOD and MDA were found in group Ⅰ and Ⅲ but obvious symptoms of kidney-yang deficiency and decrease of SOD （P〈0. 01） and increase of MDA （P〈0. 01 or P〈0. 05） were found in group Ⅲ,Ⅳ and Ⅴ ; after treatment, in the group Ⅲ the symptoms of kidney-yang deficiency basically disappeared, SOD increased （P〈0.0 1） and MDA decreased （P 〈0.01）, with no significant differences as compared with those before modeling （P〉0. 05）; in the group Ⅳ, the symptoms of kidney-yang deficiency significantly improved and SOD increased （P〈0. 05） with difference as compared with those before modeling （P〈0. 05）, and MDA decreased （P〈0. 05）, but with no significant difference compared with that before modeling （P〉0.05）; the symptoms of kidney-yang deficiency, SOD and MDA did not significantly change in group Ⅴ（P〉0. 05）. Conclusion Both moxibustion according to opening points on time in eight methods of intelligent turtle and moxibustion at Mingmen （GV 4） can effectively regulate free radical metabolism and have obvious action of preventing and treating kidney-yang deficiency in the model guinea pigs, and the therapeutic action of moxibustion according to opening points on time in eight methods of intelligent turtle is better than that of moxibustion at Mingmen （GV 4）,