摘要
目的:建立HPLC指纹图谱方法鉴别广金钱草与金钱草药材;方法:以ODS-C_(18)5μm,250mm×4.6mm色谱柱,水(A) -乙腈(B)为流动相,梯度洗脱方式:0～15min,保持85%A,15→60min,85%A→78%A,流速0.7mL·min^(-1),柱温30℃,进样量30μL,检测波长215,270,340nm;结果:广金钱草可得到以异牡荆素为参照的11个指纹峰,金钱草可得到以槲皮素为参照10个指纹峰进行比较;结论:通过指纹图谱方式,可针对性地鉴别广金钱草和金钱草。
Objective：Established a fingerprint method to identify the Herba Desmodii Styracifolii and Herba Lysi- machiae. Methods：The chromatographic column of ODS - C18 5 μm,250 mm ×4. 6 mm was used with water - acetonitrile as mobile phase by 0. 7 ml· min^-1 flow rate under 215,270 ,340 nm wavelength at 30 ℃ column temperature. The gradient elution is 0 - 15 min, keep 85% water, 15 - 60 min ,85% water→78% water. The injection volume was 30μL. Results：It compared llpeaks from Herba Desmodii Styracifolii fingerprint and 10peaks from Herba Lysimac hiae fingerprint with isovitexin and quercetin as standard respectively. Conclusion：The method can identify the Herba Desmodii Styracifolii and Herba Lysimachiae with HPLC fingerprint.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第9期1248-1250,共3页
Chinese Journal of Pharmaceutical Analysis
关键词
广金钱草
异牡荆素
金钱草
槲皮素
Herba Desmodii Styracifolii
isovitexin
Herba Lysimachiae
quercetin
