HPLC-ELSD法测定西洋参含片中人参皂苷Rg_1、Re和Rb_1的含量

HPLC-ELSD determination of ginsenosides Rg_1,Re and Rb_1 in panax buccal slices

目的:建立西洋参含片中人参皂苷Rg_1、Re、Rb_1的HPLC测定方法。方法:色谱柱:Alltech C_(18)柱(4.6mm×150mm,5μm),保护柱:Security Guard C_(18)(4mm×3.0mm),流动相:乙腈-水,梯度洗脱,流速1.0mL·min^(-1)。ELSD:雾化气体流速2.7L·min^(-1),漂移管温度115℃。样品经粉碎后,用70%甲醇水溶液超声提取30min,离心,取上清液过滤进样。结果:梯度洗脱时基线平直,线性范围:0.0100～1.00mg·mL^(-1);人参皂苷Rg_1、Re、Rb_1的相关系数分别为0.9995,0.9996,0.9986;检出限分别为40,40,25ng(S/N>3)。加样回收率:87.8%～105.2%,RSD在1.2%～4.8%之间。结论:该方法前处理简单,分析准确、快速(一次分析在40min内完成),可作为同时测定多种人参皂苷成分含量的方法。

Objective： To establish a simple,accurate method of RP -HPLC with ELSD（ evaporative light scattering detector）to determine quantitatively the three main ginsenosides Rg1, Re, Rb1 in buccal slices. Method： The method was developed with the condition as follows： Alhech C18 column （4.6 mm×150 mm,5μm）, Dikma security guard C18 （4 mm×3.0 mm）. Gradient elution was employed with the mobile phase of acetonitrile -pure water at the speed of 1.0 mL· min ^- 1. ELSD ： gas flow, 2.7 L·min^ - 1 ; temperature of the drift tube, 115 ℃. powdered sample was ultrasonically axtracted in 70% methanol for 30 min, then centrifuged and filtrated before injection. Results：The base line of the chromatogram was straight with gradient elution. The three contents were all linear over the range 0.0100 - 1.00 mg·mL^- 1. ginsenoside Rg1 ： r = 0. 9995, ginsenoside Re ： r = 0. 9996, ginsenoside Rb1： r =0.9986,and the limits of detection were 40,40,25 ng（S/N 〉3） respectively. Recoveries of the added sample was 87.8% - 105.2%. RSD was between1.2% and 4.8%. Conclusion ：With its accuracy ,sensitivity ,reproducibility as well as the simplicity of sample preparation,the method is feasible for quality control of buccal slices.