高效液相色谱法测定盐酸班布特罗胶囊的含量

HPLC Determination of the content of Banbuterol hydrochloride capsule

目的:建立测定胶囊剂中盐酸班布特罗含量的方法。方法:采用高效液相色谱法对胶囊剂中盐酸班布特罗含量进行测定。色谱条件:色谱柱Agilent Zorba×SB-C_(18),150mm×4.6mm;流动相:磷酸缓冲溶液-甲醇-乙腈(45:16:25),流速1mL·min^(-1);柱温25℃;检测波长220nm。结果:线性范围25.2～201.6μg·mL^(-1)(r=0.9999),回收率99.74%,RSD= 0.82%。结论:高效液相色谱法测定胶囊剂中盐酸班布特罗的含量方法简便易行,准确度高,重现性好,可用于控制该制剂的质量。

Objective：HPLC method to determined the content of Bambuterol hydrochloride has been established. Methods：The Agilent Zorbax SB -C18, 150 mm×4. 6 mm column was used with mobile phase phosphoric buffer solution -methanol- acetonitrile （45： 16：25 ） , the detection wavelenth was 220 nm. Result： The Linear concentration was within the range of 25.2 - 201.6μg·mL^- 1 （ r = 0. 9999 ）, and the average recovery was 99. 74% （ RSD = 0. 82%. Conclusion：The HPLC method to determined content of Banbuterol hydrochloride in capsule was simple and reliable. The method can be used to control the quality of preparation.