鲁米诺-过氧化氢-四磺基锰酞菁化学发光体系测定依诺沙星

Chemiluminescence Determination of Enoxacin with Luminol-H_2O_2-MnTSPc System

研究发现在碱性条件下,依诺沙星对Luminol-H2O2-四磺基锰钛菁(MnTSPc)体系的化学发光有较强的抑制作用,据此建立了流动注射化学发光测定依诺沙星药物的新方法。依诺沙星浓度在2.0×10-8—5.0×10-5mol/L范围内与发光信号的降低值(ΔI)呈良好的线性关系;检出限(3δ)为7.9×10-9mol/L;对于1.0×10-6mol/L依诺沙星进行11次测定,相对标准偏差为1.31%。应用于片剂中依诺沙星含量的测定,结果令人满意。

A flow injection method for the determination of enoxacm is aeveloped based, inhibiting behavior of Enoxacin on the emission intensity of the chemiluminescence reaction of Luminol-H2O2-MnTSPc in basic medium. The method is simple, selective and sensitive with a detection limit of 7. 9× 10^-9 mol/L in the range of 2. 0 ×10^-8-5. 0 × 10^-5 mol/L. The relative standard deviation is 1.31% at 1.0 × 10^-6mol/L of enoxacin concentration （n= 11）. The method was successfully applied to determination of enoxacin in tablets with satisfactory results.