摘要
通过乙醚提取样品,在C18色谱柱上(4.6×250mm,5μm)以磷酸缓冲溶液和甲醇以95∶5的比例作为流动相进行色谱分离,用HPLC法DAD225nm处检测干鱿鱼制品中的苯甲酸、山梨酸含量。本法在标准浓度为0.01—0.1mg/mL范围内,线性良好,相关系数大于0.995,该方法适合日常检测。
The samples were extracted by aether. Benzoic acid and sorbic acid in dried sleeve-fish products were determined by HPLC with DAD detector (225nm) on the C18 column(4.6 ×250mm, Stem) using elution of KH2PO4 solution and methanol (95:5) for separation. The linear range is 0.01-0. 1mg/mL with RSD〉0.995. It is suitable for daily testing.
出处
《光谱实验室》
CAS
CSCD
2006年第5期1039-1041,共3页
Chinese Journal of Spectroscopy Laboratory
关键词
HPLC法
苯甲酸
山梨酸
鱿鱼制品
HPLC, Benzoic Acid, Sorbic Acid, Sleeve-Fish Products.