DAD检测干鱿鱼制品中苯甲酸、山梨酸的含量

Determination of Benzoic Acid and Sorbic Acid in Dried Sleeve-Fish Products by HPLC with DAD Detector

通过乙醚提取样品,在C18色谱柱上(4.6×250mm,5μm)以磷酸缓冲溶液和甲醇以95∶5的比例作为流动相进行色谱分离,用HPLC法DAD225nm处检测干鱿鱼制品中的苯甲酸、山梨酸含量。本法在标准浓度为0.01—0.1mg/mL范围内,线性良好,相关系数大于0.995,该方法适合日常检测。

The samples were extracted by aether. Benzoic acid and sorbic acid in dried sleeve-fish products were determined by HPLC with DAD detector （225nm） on the C18 column（4.6 ×250mm, Stem） using elution of KH2PO4 solution and methanol （95：5） for separation. The linear range is 0.01-0. 1mg/mL with RSD〉0.995. It is suitable for daily testing.