用镱离子和邻苯二酚紫定性定量检测人体尿液和血清中的无机磷酸根

Qualitative and Quantitative Determination of Inorganic Phosphate from Human Urine and Serum by Yb(Ⅲ)Ion and Pyrocatechol Violet

用摩尔比为2∶1的YbCl3和邻苯二酚紫,在pH=7.0的HEPES缓冲溶液中,定性、定量检测人体尿液和血清中的无机磷酸根.随着尿液或经过处理后血清液的加入,在UV-Vis吸收光谱上,由623nm的最大吸收峰逐渐向444nm的最大吸收峰转移,同时体系的颜色由蓝变黄.该体系对尿液和血清中的无机磷酸根相对其它成分显示了很好的选择性.测得健康人尿液和血清中无机磷酸根的平均含量分别为23.25和1.14mmol/L,测定结果与无机磷酸根正常释放基本吻合.

Phosphate is involved in a number of important biomineralization processes such as bone formation as well as pathological processes such as the genesis of renal stones. Consequently, its determination in biological fluids is important. The determination of free phosphate ion in human urine and serum is very important, since they have diagnostic value in some clinical cases. Here we report a simple and sensitive method to determine phosphate in urine and serum. This new ensemble was prepared by adding ytterbium chloride and pyrocatechol violet in a 2：1 molar ratio in aqueous solution of 10 mmol/L HEPES buffer at pH = 7.0. With the addition of urine or serum sample, in UV-Vis absorption spectra the maximum absorption peak gradually shifts from 623 nm to 444 nm and the ensemble results in a change of color from blue to yellow. The ensemble exhibits an excellent selectivity for phosphate over other constituents of urine and serum. Mean urine and serum phosphate concentrations found in healthy individual are 23.25 and 1.14 mmol/L respectively. The detection results are basically consistent with those of normal excretion of phosphate.