大强度运动及慢性心理应激对大鼠行为、免疫细胞和皮质醇的影响

Impact on rats' behavior,immune cells and cortisol of high-intensity exercise and chronically psychological stress

目的探讨大强度运动及慢性心理应激对大鼠行为、免疫细胞和皮质醇的影响。方法在已建立的运动及心理应激动物模型上,观察大鼠在大强度运动及慢性心理应激后行为、白细胞总数和皮质醇水平的变化。结果长期大强度运动加慢性心理应激组大鼠行为明显异常。在心理应激即刻,心理应激组的WBC数明显高于大强度运动加心理应激组和大强度运动组(差异显著,P＜0．05)。慢性心理应激组在心理应激即刻皮质醇含量增加,均高于对照组和大强度运动组,差异显著(P＜0．05)；运动及心理应激后24 h,慢性心理应激组的皮质醇含量仍明显高于大强度运动组(P＜0．05)。结论(1)长期大强度运动及慢性心理应激明显改变大鼠的行为活动。(2)长期慢性心理应激对白细胞数量的变化影响较大,表明心理应激对机体的特异性免疫系统有重要影响。(3)长期慢性心理应激引起脾脏生长发育异常。(4)长期大强度运动造成皮质醇水平升高,但心理应激对皮质醇水平变化的影响更大。长期大强度运动基础上的慢性心理应激引起皮质醇水平升高不如单一的慢性心理应激明显,其可能的机理是长期大强度运动与慢性心理应激对皮质醇的影响产生交互作用。

OBJECTIVE It discusses the impact on rats＇ behavior, immune cells and cortisol of high - intensity exercise and chronically psychological stress. METHOD Based on the animal model in exercise and psychological stress, it observed the rats＇ variety of behavior, total number of leucocytes and the cortisol level after high- intensity exercise and chronically psychological stress. RESULTS The behaviors of rats in long - term and high - intensity exercise with chronically psychological stress groups were abnormal obviously. At once at psychological stress, the WBC count of chronically psychological stress group was higher than high- intensity exercise with psychological stress group and high- intensity exercise group（significant difference, P 〈 0.05） . The cortisol content of chronically psychological stress group increased at once at psychological stress, and higher than the control group and high - intensity exercise group（ significant difference, P 〈 0.05 ） ; in 24 h after psychological stress and exercise, the cortisol content of chronically psychological stress group was still higher than high- intensity exercise group obviously （P 〈 0.05 ）. CONCLUSIONS （1）The long- term and high- intensity exercise and chroni- cally psychological stress changed rats＇ behaviors significantly. （2） Chronically psychological stress exerted a great influence on change of the WBC quantity, indicating that psychological stress had an important influence on specific immune system in organism. （3） Chronically psychological stress caused abnormal development of the spleen. （4）The long- term and high - in- tensity exercise caused the cortisol level to rise, but the influence that psychological stress induced changes to the cortisol level was greater. Chronically psychological stress based on long- term and high- intensity exercise caused cortisol level to rise less significantly than single chronically psychological stress, which possible mechanism was that long- term and high- intensity exercise and chronically psychological stress generated a mutual impact on influence of cortisol.