摘要
以黑曲霉vin-1为研究对象,通过紫外诱变(UV)和硫酸二乙酯(DES)两次诱变,获得产酶较高的菌株vin-1-24-72,酶活达到527u/g,比原始菌株提高154%,RDA%值为40%。菌株经过7次传代,酶活较稳定。
A mutant strain vin-1-24-72 with high yied of raw starch-digesting glucoamylase was isolated from protoplast of Aspergillus niger vin-1 treated by UV and DES. The raw starch-digesting glucoamylase activity of the strain raised to 527 u/g, which was 154 % higher than the enzyme activity ofprotoplast strain. The RDA were 40 % .The enzyme activity of the mutant strain was very steady cultured by seven times.
出处
《酿酒科技》
北大核心
2006年第10期37-39,共3页
Liquor-Making Science & Technology
