NBS-二氯荧光素化学发光体系测定西索米星

A novel flow injection chemiluminescence method for determination of sisomicin based on NBS-dichlorofluorescein reaction

在碱性奈件下，西索米星被NBS（N-溴代琥珀酰亚胺）氧化，其能量可激发共存的二氯荧光素（DCF）而产生化学发光，发光强度与西索米星的质量浓度在一定范围内呈良好的线性关系，基于此，结合流动注射技术，建立了一种直接测定西索米星的化学发光新方法。该方法具有较高的灵敏度，检出限为73μg／L（IUPAC），线性范围为0．1～10mg／L，对5．0mg／L西索米星平行测定11次，其相对标准偏差为2．3％。已用于针剂中西索米星的测定。

A novel flow injection chemiluminescence （FI-CL） system for the determination of sisomicin was presented. It was based on the chemiluminescence from the oxidation of sisomicin by N-bromosuccinimide （NBS） in alkaline medium in the presence of dichlorofluorescein as energy transfer. The chemiluminescence intensity was proportional to sisomicin concentration over the range 0.1 - 10 mg/L. The detection limit is 73 μg/L and the relative standard deviation is 2.3 % for 5.0 mg/L Sisomicin （ n = 11）. This method had been successfully applied to the determination of sisomicin in injections.