流动注射电化学发光分析法测定维生素B_1及在尿液分析中的应用

FI chemiluminescence determination of VB_1 with on-line electrogenerated hypochlorite and its application in urine assay

将在线恒电流电解产生CIO^-与Luminol构成了较强的化学发光体系，基于维生素B1对该化学发光体系有抑制作用，结合流动注射技术，建立了测定维生素B1的流动注射化学发光新方法，并把它应用于尿液分析中。该方法在维生素B1的浓度为1．0×10^-10～1．0×10^-8mmol／mL之间分段回归，呈良好的线性，检出限达到5．0×10^-11mmol／mL。

A novel flow-injection chemiluminescence method for the determination of Vitamin B1 based on its inhibition of chemiluminescence from luminol-hypochlorite system was described. The hypochlorite was electrogenerated on-line by constant current electrolysis. The calibration curve is linear in the range of 1.0 × 10^-1^-10- 1.0× 10^-9 mmol/mL and 1.0 × 10^-9 - 1.0 × 10^-8 mmol/mL respectively with a detection limit of 5.0 × 10^-10 mmol/mL. The proposed method is reliable, accurate and very suitable for the determination of VB1 in pharmaceutical preparations. Its application in human urine assay was also described.