高效液相色谱法测定烟草料液中的糖

Analysis of sugars in tobacco sauce by high-performance liquid chromatography with evaporative light scattering detector

研究了用蒸发光散射检测器检测，高效液相色谱法测定烟草料液中糖的方法。料液中的糖用固相萃取预分离，然后以Waters carbohydrate高效糖柱为固定相，V（乙腈）：V（水）=70：30作为流动相分离，蒸发光散射检测器检测；样品中鼠李糖、木糖、阿拉伯糖、果糖、甘露糖、葡萄糖、蔗糖、麦芽糖8种糖的加标回收率分别为：97．0％、95．6％、102％、102．1％、95．0％、101．8％、102．6％、97．8％；线性范围分别为：鼠李糖、果糖、葡萄糖、蔗糖0．1～20pg，木糖、阿拉伯糖、甘露糖、麦芽糖0．2～25μg。相对标准偏差均小于3．2％。方法的检出限达：鼠李糖20ng、木糖26ng、阿拉伯糖28ng、果糖14ng、甘露糖20ng、葡萄糖10ng、蔗糖12ng、麦芽糖15ng，用该方法测定了烟草料液中的糖。

A new method for the determination of sugars （rhamnose, xylose, arobinose, fructose, mannose, glucose, sucrose, maltose） in tobacco sauce was established. The sugars in tobacco sauce were separated by solid phase extraction with Sep - pak - C18 cartridge. Then, the sugars were analysed by high performance liquid chromatography with evaporative light scattering detector. The 8 sugars were separated on high performance carbohydrate column using an V （acetonitrile） ： V（water） = 70：30 at a flow-rate of 1.0 mL/min. The recoveries were high and reproducible, ranging from 95.0% to 102.6%. This method had been successfully applied to the determination of sugars in tobacco sauce with good results.