高三酰甘油血症对大鼠胰腺腺泡细胞的影响

Effect of increased serum triglyceride level on morphology and enzymes release of rat pancreatic acinar cells.

目的研究高三酰甘油血症对大鼠胰腺腺泡细胞形态和功能的影响。方法断奶1周雄性SD大鼠(约70g)随机分为2组,每组10只,分别给予高脂饲料和普通饲料喂养4周。检测血清三酰甘油(TG)、胆固醇(TC)及游离脂肪酸(FFA)水平,HE染色和透射电镜观察胰腺腺泡细胞形态。胶原酶消化法分离大鼠胰腺腺泡细胞,台盼蓝染色测定细胞活力。给予不同浓度胆囊刺激素(CCK-8)刺激胰腺腺泡细胞分泌淀粉酶及乳酸脱氢酶(LDH),测定酶释放率。结果高脂饮食饲喂大鼠4周后,血清TG、FFA较正常饮食组明显升高(P<0.05)。高脂饮食组大鼠胰腺腺泡细胞出现空泡样改变及内质网扩张。体外培养6h后,高脂饮食组胰腺腺泡细胞活力低于对照组(P<0.05),且各浓度CCK-8引起的淀粉酶和LDH释放均高于正常饮食组(P<0.05)。结论饮食诱导的高三酰甘油血症可以引起大鼠胰腺腺泡细胞损伤。

Objective To study the effect of increased serum triglyceride levels on morphology and functions of rat pancreatic acinar cells. Methods One week after ablactation, male SD rats, weighing about 70 g, were assigned to 2 groups（n= 10）： one group was fed for 4 weeks with high fat diet and the other with normal diet. Blood samples were taken to measure triglyceride, total cholesterol, and free fatty acid in serum. Pancreatic tissues were taken for light microscope and transmission electron microscope examination. Pancreatic acinar cells were isolated by collagenase digestion and cell viability was determined hy typan blue dyeing at different time points. For measurement of amylase and LDH secretion, cells were incubated with the appropriate secretagogue for 60 min; amylase and LDH activities were measured by colorimetric assay. Results Compared with the control group, the levels of triglyceride and free fatty acid were significantly elevated in high fat diet group （P〈0.05）. Intracellular vacuole formation and dilated endoplasmic reticulum were also observed in high fat diet group. Cell viability in high fat diet group was obviously lower than that in control group after cultured in vitro for 6 h （P 〈 0.95）. Amylase and LDH release values in high fat diet group were significantly higher than those in control group at different concentrations of CCK-8（P 〈 0.05）. Conclusions The present results demonstrate that high serum triglyceride level associated with high fat diet can cause pancreatic acinar cells damage.