摘要
目的探讨急性出血坏死性胰腺炎(ANP)合并肺损伤时大鼠胰腺和肺脏组织抗凋亡因子生存素(survivin)的表达及变化情况。方法采用胆胰管逆行注射4.5%牛磺胆酸钠的方法制作大鼠ANP合并肺损伤模型。将50只Wistar大鼠随机分为对照组、造模后24h、48h、72h、96h共5组,每组10只,采用免疫组化和Westernblot法检测胰腺和肺脏中survivin的表达及变化情况。结果survivin在对照组肺组织表达呈阴性,在实验组肺泡上皮细胞、支气管上皮细胞及血管内皮细胞中表达呈阳性;在对照组胰腺组织表达呈阴性,在实验组主要表达于胰腺导管细胞的胞核和胞质,腺泡细胞胞核中有散在表达,胞质中未见表达。Westernblot结果显示,对照组肺和胰腺组织无survivin蛋白带,实验组肺脏和胰腺组织中表达逐渐增强,48h达高峰,96h下降明显。结论survivin在ANP组织中及合并肺损伤的肺组织中异常高表达,可能起到减轻肺损伤、促进修复的作用。
Objective To investigate the changes of survivin in the pancreatic and pulmonary tissues of rats with acute necrohemorrhagie panereatitis eomplieated with lung injury. Methods The acute necrohemorrhagie panereatitis complicated （ANP） with lung injury model was induced by bilipanereatitie duct infusion with 4. 5% sterile sodium taurocholate solution. Fifty Wistar rats were randomly divided into 5 groups （n =10）： control group, post-infusion 24 h group, 48 h group, 72 h group, and 96 h group. The proteins of survivin were detected by irnmunohistoehemistry and Western blot analysis in the lung and pancreas. Results Immunohistoehemistry results showed that there was no survivin expression in the lung of control group. After induction of acute neerohemorrhagie panereathis, survivin protein was found in alveolar epithelia, bronehia epithelia and vascular endothelia, survivin expression was not observed in the pancreas of the control group. After induction of acute neerohemorrhagie panereatitis, survivin protein was mainly found in duetal cells and a few in aciner cells. Western blot showed there was no surviving protein bands in the lung and pancreas of control group, while the expression of survivin in ANP model group gradually increased as time went by, peaked at 48 h, and decreased at 96 h. Conclusions survivin is highly expressed in the pancreatic and pulmonary tissues of rats with ANP and lung injury, which may play a role in relieving and repair of the lung injury.
出处
《胰腺病学》
2006年第5期285-288,共4页
Chinese JOurnal of Pancreatology
