银屑病患者CD147、亲环素A和亲环素B表达

Expression of CD147,cyclophilin A and cyclophilin B in patients with psoriasis

目的探讨CD147、亲环素A和亲环素B存银屑病发病机制中的作用。方法用RT-PCR半定量检测40例寻常性银屑病、15例脓疱性银屑病患者和20例正常人外周血T细胞和中性粒细胞中CD147、亲环素A和亲环素B mRNA的表达水平。结果在T细胞中,寻常性银屑病组CD147、亲环素A和亲环素B mRNA表达水平分别为1．231±0．128、1．254±0．096和1．667±0．166,显著高于正常人对照组(分别为0．983±0．105、1．084±0．070和1．386±0．152,P值均＜0．01),且患者组三者表达水平均与其相应的PASI积分呈正相关(r值分别为0．544、0．643和0．668,P值均＜0．01)；脓疱性银屑病组三者表达水平分别为1．418±0．117、1．760±0．160和1．959±0．156,显著高于寻常性组(P值均＜0．01)。寻常性银屑病组中性粒细胞CD147 mRNA表达水平(2．118±0．278)显著高于正常人对照组(1．393±0．144,P＜0．01),且与其相应的PASI积分呈正相关(r值为0．618,P＜0．01)；脓疱性银屑病组中性粒细胞CD147的表达水平(3．072±0．371)显著高于寻常性银屑病组(P＜0．01)。寻常性银屑病组亲环素A和亲环素B mRNA表达水平分别为1．127±0．086和1．081±0．124,与正常人对照组比较差异无统计学意义(P值均＞0．05)；并与其相应的PASI积分无线性相关关系(r值分别为0．290和0．144,P值均＞0．05)。脓疱性银屑病组二者的表达水平(分别为1．116±0．075和1．096±0．133)与寻常性银倩病组比较差异无统计学意义(P值均＞0．05)。结论CD147、亲环素A和亲环素B可能在银屑病疾病活动和发展过程中起作用。

Objective To investigate the roles of CD147, cyclophilin A （ CyPA ） and cyclophilin B （ CyPB ） in the pathogenesis of psoriasis. Methods Reverse transcriptase-polymerase chain reaction （RT-PCR） was used to semi-quantitatively measure the mRNA expression of CD147, CyPA and CyPB in peripheral blood T cells and neutrophils from 40 patients with psoriasis vulgaris, 15 patients with psoriasis pustulosa and 20 normal controls. Results The mRNA expression levels of CD 147, CyPA and CyPB in T cells from patients with psoriasis vulgaris were significantly higher than those from normal controls （ 1.231 ± 0.128 vs 0.983 ± 0.105, 1.254 ± 0.096 vs 1.084 ± 0.070, 1.667 ± 0.166 vs 1.386 ± 0.152, respectively, all P〈 0.01 ）, but significantly lower than those from patients with psoriasis pustulosa （ 1.231 ± 0.127 vs 1.418 ± 0.117, 1.254 ± 0.095 vs 1.760 ± 0.160, 1.667 ± 0.165 vs 1.959 ± 0.156, respectively, all P 〈 0.01 ）, and they were positively correlated with the corresponding psoriasis area and severity indix （ PASI ） score （ r= 0.544, 0.643, 0.668 respectively, all P〈 0.01 ）. The mRNA expression level of CD147 in neutrophils from patients with psoriasis vulgaris was significantly higher than that from normal controls （2.118 ± 0.278 vs 1.393 ± 0.144, P〈 0.01 ）, but lower than that from patients with psoriasis pustulosa （ 2.118 ± 0.278 vs 3.072 ± 0.371, P 〈 0.01 ）, and it also positively correlated with the corresponding PASI score （ r= 0.618, P〈 0.01 ）. There was no statistical difference between the mRNA expression levels of CyPA and CyPB in neutrophils from the patients with psoriasis vulgaris and those from the normal controls （ 1.127 ± 0.086 vs 1.253±0.111, 1.081 ± 0.124 vs 1.067±0.134 respectively, both P〉 0.05） or those from the patients with psoriasis pustulosa （ 1.127 ± 0.086 vs 1.116 ± 0.075, 1.081 ± 0.124 vs 1.096 ± 0.133 respectively, both P〉 0.05 ）, and no correlation was found between them and the corresponding PASI score （ r= 0.290 and 0.144 respectively, both P〉 0.05 ）. Conclusion CD147, CyPA and CyPB might play a role in the disease activity of and progress in psoriasis.