荞麦组织培养及高频植株再生体系的建立

TISSUE CULTURE AND HIGH-FREQUENCY PLANT REGENERATION OF BUCKWHEAT (FAGOPYRUM ESCULENTUM MOENCH)

通过对荞麦(Fagopyrum esculentum Moench)不同外植体、不同激素配比的比较研究,建立了荞麦离体培养高效植株再生体系。荞麦子叶切段在含2．0 mg/L 2,4-D和1．0 mg/L 6-BA的MS培养基上愈伤组织诱导率为89．6％,而下胚轴切段在含2．0 mg/L 2,4-D和1．0-2．0 mg/L 6-BA MS培养基上愈伤组织诱导率高达100％。在2．0 mg／L 6-BA、0．1 mg,L IAA和1 mg／L KT的MS培养基上通过愈伤组织间接分化或外植体直接分化形成不定芽。来自子叶和下胚轴的愈伤组织的分化率分别为42．5％和73．6％,下胚轴的分化率明显高于子叶。将生长状态良好的不定芽转至含1．0 mg/L IBA和0．5mg/L NAA的1/2 MS培养基上生根,生根率达到100％。再生植株移栽到盆土中,成活率达91．6％,并且生长状态和特征均表现正常。

Different explants and compounding proportions of different hormones were comparatively studied in tissue culture of Fagopyrum esculentum Moench and thereafter an efficient plant -regeneration system was established by in vitro F.esculentum Moench culture. On MS medium containing 2.0 mg/L 2,4-D and 1.0 mg/L 6-BA,89.6% cotyledon segments could be induced to produce calli; however,on MS medium containing 2.0 mg/L 2,4-D and 1.0-2.0 mg/L 6-BA,the induction rate of hypocotyl segments could reach as high as 100%. On MS medium containing 2.0 mg/L 6-BA,0.1 mg/L IAA and 1 mg/L KT,adventitious buds could be regenerated indirectly from calli or directly from explants,the differentiation rates of cotyledon-derived calli and hypocotyl-derived calli were 42.5% and 73.6% respectively; the calli coming from hypocotyl segments differentiated evidently at a higher rate than the calli originated from cotyledon segments. Well-grown adventitious buds were inoculated on 1/2 MS medium containing 1.0 mg/L IBA and 0.5mg/L NAA,100% rooting frequency was obtained. Plantlets grew well and appeared normal with no mortality after being transplanted to soil. Moreover,the survival rate of plantlets reached 91.6%.